PFKP Antibody (F-7): sc-514824

PFKP Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects PFKP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-PFKP antibody (F-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PFKP, also known as phosphofructokinase platelet, is a critical component of the glycolytic pathway, functioning as an allosteric enzyme that catalyzes the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate, a key regulatory step in glucose metabolism. PFKP is primarily located in platelets, where PFKP plays a vital role in energy production and metabolic regulation, particularly during periods of increased energy demand. Proper functioning of PFKP is essential for maintaining cellular energy homeostasis, and any defects in PFKP gene can lead to metabolic disorders, including an increased risk of obesity. Using PFKP (F-7) monoclonal antibody in your research can provide valuable insights into PFKP′s role in various physiological and pathological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PFKP Antibody (F-7) References:

1. Trifunctional chemical probes for the consolidated detection and identification of enzyme activities from complex proteomes.  |  Adam, GC., et al. 2002. Mol Cell Proteomics. 1: 828-35. PMID: 12438565
2. Cloning and chromosomal characterization of the 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 gene (PFKFB3, iPFK2).  |  Mahlknecht, U., et al. 2003. Int J Oncol. 23: 883-91. PMID: 12963966
3. Characterization of the human P-type 6-phosphofructo-1-kinase gene promoter in neural cell lines.  |  Hannemann, A., et al. 2005. Gene. 345: 237-47. PMID: 15716112
4. Chimeric phosphofructokinases involving exchange of the N- and C-terminal halves of mammalian isozymes: implications for ligand binding sites.  |  Martínez-Costa, OH., et al. 2007. FEBS Lett. 581: 3033-8. PMID: 17544406
5. Isolation and sequence of a cDNA encoding human platelet phosphofructokinase.  |  Simpson, CJ. and Fothergill-Gilmore, LA. 1991. Biochem Biophys Res Commun. 180: 197-203. PMID: 1834056
6. Non-replication of genome-wide based associations between common variants in INSIG2 and PFKP and obesity in studies of 18,014 Danes.  |  Andreasen, CH., et al. 2008. PLoS One. 3: e2872. PMID: 18682847
7. Cloning of human muscle phosphofructokinase cDNA.  |  Nakajima, H., et al. 1987. FEBS Lett. 223: 113-6. PMID: 2822475
8. Prognostic and therapeutic relevance of phosphofructokinase platelet-type (PFKP) in breast cancer.  |  Umar, SM., et al. 2020. Exp Cell Res. 396: 112282. PMID: 32919954
9. p53-responsive CMBL reprograms glucose metabolism and suppresses cancer development by destabilizing phosphofructokinase PFKP.  |  Huang, Y., et al. 2023. Cell Rep. 42: 113426. PMID: 37967006
10. Inhibition of PFKP in renal tubular epithelial cell restrains TGF-β induced glycolysis and renal fibrosis.  |  Yang, S., et al. 2023. Cell Death Dis. 14: 816. PMID: 38086793
11. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.  |  Cheng, Z., et al. 2024. iScience. 27: 108645. PMID: 38155775
12. Isozymes of human phosphofructokinase: identification and subunit structural characterization of a new system.  |  Vora, S., et al. 1980. Proc Natl Acad Sci U S A. 77: 62-6. PMID: 6444721

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PFKP Antibody (F-7) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-PFKP Antibody (F-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PFKP Antibody (F-7) Specifications

Alternate Product Name: PFKP

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 131-174

Epitope Target: PFKP

Epitope Species: human

Product Reactivity/Detection: PFKP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for PFKP Antibody (F-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin