PDE1B Antibody (D-11): sc-393349

PDE1B Antibody (D-11) is a mouse monoclonal IgG1 antibody that detects PDE1B in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PDE1B is a critical enzyme in the regulation of cyclic nucleotide signaling, specifically hydrolyzing cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) into their respective nucleoside 5-prime-monophosphates, thereby influencing various physiological processes including smooth muscle tone and neuronal signaling. The importance of PDE1B′s function is underscored by its role in modulating intracellular calcium levels through calmodulin-dependent activity, which is essential for processes such as learning and memory in the central nervous system. The human PDE1B gene, located on chromosome 12q13, encodes a 536-amino acid protein that is expressed in key neuronal regions such as the cerebellum and hippocampus, as well as in lymphoblastoid cell lines. Notably, PDE1B is involved in the phosphorylation regulation of DARPP-32 in dopaminergic neurons, linking PDE1B to critical neurobiological functions. Additionally, a splice variant, PDE1B2, produces a protein that differs from PDE1B1 by substituting the first 38 amino acids with an alternative sequence, highlighting the complexity and regulatory potential of this enzyme. Anti-PDE1B antibody (D-11) is an invaluable tool for researchers investigating the intricate roles of cyclic nucleotide signaling in health and disease.

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PDE1B Antibody (D-11) References:

1. Phosphodiesterase 1B knock-out mice exhibit exaggerated locomotor hyperactivity and DARPP-32 phosphorylation in response to dopamine agonists and display impaired spatial learning.  |  Reed, TM., et al. 2002. J Neurosci. 22: 5188-97. PMID: 12077213
2. Phosphodiesterase 1B differentially modulates the effects of methamphetamine on locomotor activity and spatial learning through DARPP32-dependent pathways: evidence from PDE1B-DARPP32 double-knockout mice.  |  Ehrman, LA., et al. 2006. Genes Brain Behav. 5: 540-51. PMID: 17010100
3. Phosphodiesterase inhibitors as potential cognition enhancing agents.  |  Schmidt, CJ. 2010. Curr Top Med Chem. 10: 222-30. PMID: 20166957
4. Phosphodiesterase-1b deletion confers depression-like behavioral resistance separate from stress-related effects in mice.  |  Hufgard, JR., et al. 2017. Genes Brain Behav. 16: 756-767. PMID: 28488329
5. Novel selective PDE type 1 inhibitors cause vasodilatation and lower blood pressure in rats.  |  Laursen, M., et al. 2017. Br J Pharmacol. 174: 2563-2575. PMID: 28548283
6. Phosphodiesterase type 1 inhibition alters medial prefrontal cortical activity during goal-driven behaviour and partially reverses neurophysiological deficits in the rat phencyclidine model of schizophrenia.  |  Hayes, J., et al. 2021. Neuropharmacology. 186: 108454. PMID: 33444639
7. miR-5701 promoted apoptosis of clear cell renal cell carcinoma cells by targeting phosphodiesterase-1B.  |  Zhao, C., et al. 2021. Anticancer Drugs. 32: 855-863. PMID: 33929990
8. High-Throughput Sequencing Data Reveal an Antiangiogenic Role of HNF4A-Mediated CACNA1A/VEGFA Axis in Proliferative Diabetic Retinopathy.  |  Yin, Y., et al. 2023. Invest Ophthalmol Vis Sci. 64: 32. PMID: 37342032
9. Unique transcriptional signatures correlate with behavioral and psychological symptom domains in Alzheimer's disease.  |  Fisher, DW., et al. 2024. Transl Psychiatry. 14: 178. PMID: 38575567
10. Novel Tryptophan Hydroxylase Inhibitor TPT-001 Reverses PAH, Vascular Remodeling, and Proliferative-Proinflammatory Gene Expression.  |  Legchenko, E., et al. 2024. JACC Basic Transl Sci. 9: 890-902. PMID: 39170954
11. Structural insight into the lead identification of a dual inhibitor of PDE1B and PDE10A: Integrating pharmacophore-based virtual screening, molecular docking, and structure-activity-relationship approaches.  |  Soon, CW., et al. 2024. Heliyon. 10: e38305. PMID: 39391487
12. Genomic structure and chromosome location of the murine PDE1B phosphodiesterase gene.  |  Reed, TM., et al. 1998. Mamm Genome. 9: 571-6. PMID: 9657856

The preferred PDE1B antibody is PDE1B Antibody (G-5): sc-393112.