p53 Antibody (Pab 1801): sc-98
- p53 Antibody (Pab 1801) is a mouse monoclonal IgG1 κ, cited in 412 publications, provided at 200 µg/ml
- N-terminal epitope mapping between amino acids 32-79 of p53 of human origin
- Anti-p53 Antibody (Pab 1801) is recommended for detection of p53 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
- p53 Antibody (Pab 1801) is available conjugated to agarose for IP; and to phycoerythrin for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-98 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for p53 Antibody (Pab 1801) for WB and IHC(P) applications. These reagents are now offered in bundles with p53 Antibody (Pab 1801) (see ordering information below).
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p53 Antibody (Pab 1801) is an IgG1 kappa mouse monoclonal p53 antibody (also designated TP53 antibody) that detects p53 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. p53 Antibody (Pab 1801) is available as both conjugated and non-conjugated anti-p53 antibody forms. p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p53 Antibody (Pab 1801) References:
- P53 regulation and function in normal cells and tumors. | Liu, Y. and Kulesz-Martin, M. 2000. Medicina (B Aires). 60 Suppl 2: 9-11. PMID: 11188940
- p53 protein regulates the effects of amifostine on apoptosis, cell cycle progression, and cytoprotection. | Lee, EJ., et al. 2003. Br J Cancer. 88: 754-9. PMID: 12618886
- DNA-protein crosslinks and p53 protein expression in relation to occupational exposure to formaldehyde. | Shaham, J., et al. 2003. Occup Environ Med. 60: 403-9. PMID: 12771391
- [Relevance of p53 protein and its mutations for novel strategies in cancer therapy]. | Müller, P., et al. 2004. Cas Lek Cesk. 143: 313-7. PMID: 15305766
- Regulation of p53--insights into a complex process. | Boehme, KA. and Blattner, C. 2009. Crit Rev Biochem Mol Biol. 44: 367-92. PMID: 19929178
- p53 and its mutants in tumor cell migration and invasion. | Muller, PA., et al. 2011. J Cell Biol. 192: 209-18. PMID: 21263025
- Chaperoning of mutant p53 protein by wild-type p53 protein causes hypoxic tumor regression. | Gogna, R., et al. 2012. J Biol Chem. 287: 2907-14. PMID: 22147694
- p53 protein isoforms: key regulators in the front line of pathogen infections? | Terrier, O., et al. 2013. PLoS Pathog. 9: e1003246. PMID: 23592981
- Case Study: discovery of inhibitors of the MDM2-p53 protein-protein interaction. | Liu, L., et al. 2015. Methods Mol Biol. 1278: 567-85. PMID: 25859977
- A role for bioinorganic chemistry in the reactivation of mutant p53 in cancer. | Miller, JJ., et al. 2022. J Biol Inorg Chem. 27: 393-403. PMID: 35488931
- The DNA binding activity of wild type p53 is modulated by blocking its various antigenic epitopes. | Wolkowicz, R., et al. 1995. Oncogene. 10: 1167-74. PMID: 7535417
- [p53]. | Takahashi, R. 1997. Gan To Kagaku Ryoho. 24: 1381-5. PMID: 9309129
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p53 Antibody (Pab 1801) | sc-98 | 200 µg/ml | $322.00 | |||
p53 Antibody (Pab 1801): m-IgG Fc BP-HRP Bundle | sc-526446 | 200 µg Ab; 10 µg BP | $361.00 | |||
p53 Antibody (Pab 1801): m-IgG1 BP-HRP Bundle | sc-531819 | 200 µg Ab; 20 µg BP | $361.00 | |||
p53 Antibody (Pab 1801) X | sc-98 X | 200 µg/0.1 ml | $322.00 | |||
p53 Antibody (Pab 1801) AC | sc-98 AC | 500 µg/ml, 25% agarose | $424.00 | |||
p53 Antibody (Pab 1801) PE | sc-98 PE | 200 µg/ml | $349.00 | |||
p53 Antibody (Pab 1801) Alexa Fluor® 488 | sc-98 AF488 | 200 µg/ml | $364.00 | |||
p53 Antibody (Pab 1801) Alexa Fluor® 546 | sc-98 AF546 | 200 µg/ml | $364.00 | |||
p53 Antibody (Pab 1801) Alexa Fluor® 594 | sc-98 AF594 | 200 µg/ml | $364.00 | |||
p53 Antibody (Pab 1801) Alexa Fluor® 647 | sc-98 AF647 | 200 µg/ml | $364.00 | |||
p53 Antibody (Pab 1801) Alexa Fluor® 680 | sc-98 AF680 | 200 µg/ml | $364.00 | |||
p53 Antibody (Pab 1801) Alexa Fluor® 790 | sc-98 AF790 | 200 µg/ml | $364.00 | |||
p53 Antibody (Pab 1801) Alexa Fluor® 405 | sc-98 AF405 | 200 µg/ml | $364.00 |