p130 Cas Antibody (C-4): sc-365200

p130 Cas Antibody (C-4) is a mouse monoclonal IgG1 antibody that detects p130 Cas in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p130 Cas, also known as Crk-associated substrate, plays a crucial role in cellular signaling pathways, particularly in cell adhesion and migration. p130 Cas is primarily located in the cytoplasm but can translocate to the cell membrane upon tyrosine phosphorylation, which is essential for mediating signals from growth factor receptors and integrins. p130 Cas relocates to allow dynamic regulation of signaling pathways that control cellular responses to external stimuli, such as proliferation and differentiation. p130 Cas contains multiple SH2-binding motifs, making p130 Cas a key substrate for various protein tyrosine kinases, including v-Src and v-Crk, which influence phosphorylation status and subsequent cellular localization. p130 Cas monoclonal antibody (C-4) serves as an invaluable tool for researchers studying cell signaling mechanisms and p130 Cas functions in various biological processes.

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p130 Cas Antibody (C-4) References:

1. Identification of p130cas as an in vivo substrate of receptor protein-tyrosine phosphatase alpha.  |  Buist, A., et al. 2000. J Biol Chem. 275: 20754-61. PMID: 10787408
2. p130Cas regulates the activity of AND-34, a novel Ral, Rap1, and R-Ras guanine nucleotide exchange factor.  |  Gotoh, T., et al. 2000. J Biol Chem. 275: 30118-23. PMID: 10896938
3. Serine phosphorylation of focal adhesion kinase in interphase and mitosis: a possible role in modulating binding to p130(Cas).  |  Ma, A., et al. 2001. Mol Biol Cell. 12: 1-12. PMID: 11160818
4. Y-27632, an inhibitor of Rho-associated kinases, prevents tyrosine phosphorylation of focal adhesion kinase and paxillin induced by bombesin: dissociation from tyrosine phosphorylation of p130(CAS).  |  Sinnett-Smith, J., et al. 2001. Exp Cell Res. 266: 292-302. PMID: 11399057
5. Fluoroaluminate stimulates phosphorylation of p130 Cas and Fak and increases attachment and spreading of preosteoblastic MC3T3-E1 cells.  |  Freitas, F., et al. 2002. Bone. 30: 99-108. PMID: 11792571
6. Synergistic promotion of c-Src activation and cell migration by Cas and AND-34/BCAR3.  |  Riggins, RB., et al. 2003. J Biol Chem. 278: 28264-73. PMID: 12740391
7. Blockade of v-Src-stimulated tumor formation by the Src homology 3 domain of Crk-associated substrate (Cas).  |  Cheng, CH., et al. 2004. FEBS Lett. 557: 221-7. PMID: 14741371
8. A critical role for p130Cas in the progression of pulmonary hypertension in humans and rodents.  |  Tu, L., et al. 2012. Am J Respir Crit Care Med. 186: 666-76. PMID: 22798315
9. Complexes of focal adhesion kinase (FAK) and Crk-associated substrate (p130(Cas)) are elevated in cytoskeleton-associated fractions following adhesion and Src transformation. Requirements for Src kinase activity and FAK proline-rich motifs.  |  Polte, TR. and Hanks, SK. 1997. J Biol Chem. 272: 5501-9. PMID: 9038154
10. Growth hormone stimulates the formation of a multiprotein signaling complex involving p130(Cas) and CrkII. Resultant activation of c-Jun N-terminal kinase/stress-activated protein kinase (JNK/SAPK).  |  Zhu, T., et al. 1998. J Biol Chem. 273: 33864-75. PMID: 9837978

The preferred p130 Cas antibody is p130 Cas Antibody (35B.1A4): sc-20029.