p-GSK3 beta Antibody (3A8): sc-81495

p-GSK-3β Antibody (3A8) is a mouse monoclonal IgG1 antibody that detects glycogen synthase kinase-3β (GSK-3β) in mouse, rat, human, and canine samples through western blotting (WB) and immunoprecipitation (IP) applications. anti-GSK-3β antibody (3A8) specifically recognizes the phosphorylated form of GSK-3β, which plays a crucial role in various cellular processes, including energy metabolism, cell proliferation, and apoptosis regulation. GSK-3β is primarily located in the cytoplasm but can translocate to the nucleus, where GSK-3β influences the activity of transcription factors and other proteins involved in cell signaling pathways. This subcellular localization is vital because GSK-3β modulates the activity of key proteins that govern cellular responses to growth factors and stress signals. For instance, GSK-3β′s nuclear activity is essential for the anti-apoptotic response mediated by Rel dimers in response to tumor necrosis factor-alpha (TNFα), highlighting GSK-3β′s importance in cell survival and differentiation. The phosphorylation state of GSK-3β, regulated by various kinases, determines GSK-3β′s activity and interaction with other proteins, making GSK-3β a critical player in maintaining cellular homeostasis. GSK-3β monoclonal antibody (3A8) enables researchers to gain insights into the regulatory mechanisms of GSK-3β and its implications in diseases such as cancer and neurodegenerative disorders.

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p-GSK3 beta Antibody (3A8) References:

1. The structure of phosphorylated GSK-3beta complexed with a peptide, FRATtide, that inhibits beta-catenin phosphorylation.  |  Bax, B., et al. 2001. Structure. 9: 1143-52. PMID: 11738041
2. 14-3-3 binds to and mediates phosphorylation of microtubule-associated tau protein by Ser9-phosphorylated glycogen synthase kinase 3beta in the brain.  |  Yuan, Z., et al. 2004. J Biol Chem. 279: 26105-14. PMID: 15073173
3. Adhesion molecules alpha, beta and gamma-catenin as prognostic factors of tumour progression in upper urinary tract urothelial tumours: the role of AKT-P/GSK-3beta/beta-catenin pathway.  |  Izquierdo, L., et al. 2009. BJU Int. 104: 100-6. PMID: 19154502
4. Age-related changes of Nrf2 and phosphorylated GSK-3β in a mouse model of accelerated aging (SAMP8).  |  Tomobe, K., et al. 2012. Arch Gerontol Geriatr. 54: e1-7. PMID: 21784539
5. Antisense oligonucleotide against GSK-3β in brain of SAMP8 mice improves learning and memory and decreases oxidative stress: Involvement of transcription factor Nrf2 and implications for Alzheimer disease.  |  Farr, SA., et al. 2014. Free Radic Biol Med. 67: 387-95. PMID: 24355211
6. Lithium increases platelet serine-9 phosphorylated GSK-3β levels in drug-free bipolar disorder during depressive episodes.  |  de Sousa, RT., et al. 2015. J Psychiatr Res. 62: 78-83. PMID: 25691093
7. Transient Cerebral Ischemia Alters GSK-3β and p-GSK-3β Immunoreactivity in Pyramidal Neurons and Induces p-GSK-3β Expression in Astrocytes in the Gerbil Hippocampal CA1 Area.  |  Chen, BH., et al. 2017. Neurochem Res. 42: 2305-2313. PMID: 28349361
8. Curcumin ameliorates scopolamine-induced mice memory retrieval deficit and restores hippocampal p-Akt and p-GSK-3β.  |  SoukhakLari, R., et al. 2018. Eur J Pharmacol. 841: 28-32. PMID: 30321530
9. A phospholipase D2 inhibitor, CAY10594, ameliorates acetaminophen-induced acute liver injury by regulating the phosphorylated-GSK-3β/JNK axis.  |  Lee, SK., et al. 2019. Sci Rep. 9: 7242. PMID: 31076618
10. Phosphorylated GSK‑3β protects stress‑induced apoptosis of myoblasts via the PI3K/Akt signaling pathway.  |  Liu, M., et al. 2020. Mol Med Rep. 22: 317-327. PMID: 32377749

The preferred GSK3 alpha/beta antibody is GSK3 alpha/beta Antibody (0011-A): sc-7291.