p-GM130 Antibody (C-11): sc-377549
- p-GM130 Antibody (C-11) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- raised against a short amino acid sequence containing Ser 25 phosphorylated GM130 of human origin
- Anti-p-GM130 Antibody (C-11) is recommended for detection of Ser 25 phosphorylated GM130 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including bovine
- Anti-p-GM130 Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for p-GM130 Antibody (C-11) for WB applications. These reagents are now offered in bundles with p-GM130 Antibody (C-11) (see ordering information below).
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SEE ALSO...
p-GM130 Antibody (C-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ser 25 phosphorylated GM130 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p-GM130 (C-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GM130, a critical cis-Golgi matrix protein, plays a vital role in organizing and maintaining Golgi apparatus function, which is essential for proper protein processing and trafficking within cells. GM130′s structure includes a unique amino-terminus that binds to vesicle docking protein p115, while its carboxy-terminus anchors to Golgi membranes, facilitating transport vesicle tethering. This interaction enables protein transport from endoplasmic reticulum to Golgi, ensuring correct protein modification and sorting. Phosphorylation of GM130 at Serine 25 during mitosis, mediated by Cdc2, disrupts p115 binding, leading to Golgi apparatus fragmentation - a key event in cell division. Understanding GM130 dynamics and interactions helps elucidate intracellular transport mechanisms and Golgi function regulation, making anti-GM130 antibody (C-11) an invaluable research tool.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p-GM130 Antibody (C-11) References:
- The role of the tethering proteins p115 and GM130 in transport through the Golgi apparatus in vivo. | Seemann, J., et al. 2000. Mol Biol Cell. 11: 635-45. PMID: 10679020
- Binding relationships of membrane tethering components. The giantin N terminus and the GM130 N terminus compete for binding to the p115 C terminus. | Linstedt, AD., et al. 2000. J Biol Chem. 275: 10196-201. PMID: 10744704
- The mitotic phosphorylation cycle of the cis-Golgi matrix protein GM130. | Lowe, M., et al. 2000. J Cell Biol. 149: 341-56. PMID: 10769027
- The p115-interactive proteins GM130 and giantin participate in endoplasmic reticulum-Golgi traffic. | Alvarez, C., et al. 2001. J Biol Chem. 276: 2693-700. PMID: 11035033
- Determination of functional regions of p125, a novel mammalian Sec23p-interacting protein. | Mizoguchi, T., et al. 2000. Biochem Biophys Res Commun. 279: 144-9. PMID: 11112430
- Coordination of golgin tethering and SNARE assembly: GM130 binds syntaxin 5 in a p115-regulated manner. | Diao, A., et al. 2008. J Biol Chem. 283: 6957-67. PMID: 18167358
- Mitotic Golgi vesiculation involves mechanisms independent of Ser25 phosphorylation of GM130. | Sundaramoorthy, S., et al. 2010. Cell Cycle. 9: 3100-5. PMID: 20699666
- Cdk1 protects against oxygen-glucose deprivation and reperfusion-induced Golgi fragmentation and apoptosis through mediating GM130 phosphorylation. | Li, Z., et al. 2023. J Mol Histol. 54: 609-619. PMID: 37831422
- The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner. | Nakamura, N., et al. 1997. Cell. 89: 445-55. PMID: 9150144
- Cdc2 kinase directly phosphorylates the cis-Golgi matrix protein GM130 and is required for Golgi fragmentation in mitosis. | Lowe, M., et al. 1998. Cell. 94: 783-93. PMID: 9753325
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-GM130 Antibody (C-11) | sc-377549 | 200 µg/ml | $322.00 | |||
p-GM130 Antibody (C-11): m-IgG Fc BP-HRP Bundle | sc-527609 | 200 µg Ab; 10 µg BP | $361.00 | |||
p-GM130 Antibody (C-11): m-IgG1 BP-HRP Bundle | sc-532982 | 200 µg Ab; 20 µg BP | $361.00 | |||
p-GM130 Antibody (C-11) AC | sc-377549 AC | 500 µg/ml, 25% agarose | $424.00 | |||
p-GM130 Antibody (C-11) HRP | sc-377549 HRP | 200 µg/ml | $322.00 | |||
p-GM130 Antibody (C-11) FITC | sc-377549 FITC | 200 µg/ml | $336.00 | |||
p-GM130 Antibody (C-11) PE | sc-377549 PE | 200 µg/ml | $349.00 | |||
p-GM130 Antibody (C-11) Alexa Fluor® 488 | sc-377549 AF488 | 200 µg/ml | $364.00 | |||
p-GM130 Antibody (C-11) Alexa Fluor® 546 | sc-377549 AF546 | 200 µg/ml | $364.00 | |||
p-GM130 Antibody (C-11) Alexa Fluor® 594 | sc-377549 AF594 | 200 µg/ml | $364.00 | |||
p-GM130 Antibody (C-11) Alexa Fluor® 647 | sc-377549 AF647 | 200 µg/ml | $364.00 | |||
p-GM130 Antibody (C-11) Alexa Fluor® 680 | sc-377549 AF680 | 200 µg/ml | $364.00 | |||
p-GM130 Antibody (C-11) Alexa Fluor® 790 | sc-377549 AF790 | 200 µg/ml | $364.00 | |||
p-GM130 (C-11) Neutralizing Peptide | sc-377549 P | 100 µg/0.5 ml | $69.00 |