p-Akt1/2/3 Antibody (A-12): sc-271964
- p-Akt1/2/3 Antibody (A-12) is a mouse monoclonal IgM κ, cited in 19 publications, provided at 200 µg/ml
- epitope corresponding to a short amino acid sequence containing phosphorylated Thr 308/309/305 of Akt2 of human origin
- recommended for detection of Thr 308 phosphorylated Akt1 and correspondingly Thr 309 phosphorylated Akt2 and correspondingly Thr 305 phosphorylated Akt3 of mouse, rat, human and origin by WB, IP, IF and ELISA; also reactive with additional species, including equine and avian
- m-IgGκ BP-HRP is the preferred secondary detection reagent for p-Akt1/2/3 Antibody (A-12) for WB applications. This reagent is now offered in a bundle with p-Akt1/2/3 Antibody (A-12) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
p-Akt1/2/3 Antibody (A-12) is a mouse monoclonal IgM antibody that detects the phosphorylated form of the Akt1, Akt2, and Akt3 proteins, specifically targeting a sequence containing phosphorylated Thr 308 of Akt2 in mouse, rat, and human samples. The Akt family, also known as protein kinase B isoforms, plays a central role in cellular processes such as metabolism, cell survival, and growth regulation. Upon phosphorylation at Thr 308 and Ser 473, p-Akt isoforms become fully activated and translocate to the plasma membrane, enabling their interaction with key signaling proteins like phosphoinositide-dependent kinase-1 (PDK1), mammalian target of rapamycin complex 2 (mTORC2), and glycogen synthase kinase-3 beta (GSK-3β). These interactions regulate critical pathways involved in glucose metabolism, protein synthesis, and apoptosis. Akt2, which is predominantly associated with insulin signaling, also interacts with AS160 and FOXO transcription factors to control glucose uptake and storage. Dysregulated p-Akt signaling contributes to pathological conditions, including cancer, metabolic disorders, and insulin resistance. Anti-p-Akt1/2/3 antibody (A-12) is recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), enabling researchers to study activation dynamics and interaction networks of p-Akt1/2/3. This makes p-Akt1/2/3 (A-12) antibody a valuable tool for understanding the molecular mechanisms underlying Akt-mediated signaling pathways in both normal and diseased states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p-Akt1/2/3 Antibody (A-12) References:
- Optimization of 2,3,5-trisubstituted pyridine derivatives as potent allosteric Akt1 and Akt2 inhibitors. | Hartnett, JC., et al. 2008. Bioorg Med Chem Lett. 18: 2194-7. PMID: 18294842
- Identification and quantification of AKT isoforms and phosphoforms in breast cancer using a novel nanofluidic immunoassay. | Iacovides, DC., et al. 2013. Mol Cell Proteomics. 12: 3210-20. PMID: 23929892
- Prolactin modulates cytokine production induced by culture filtrate proteins of M. bovis through different signaling mechanisms in THP1 cells. | Martínez-Neri, PA., et al. 2015. Cytokine. 71: 38-44. PMID: 25218920
- Stimulus-dependent differences in signalling regulate epithelial-mesenchymal plasticity and change the effects of drugs in breast cancer cell lines. | Cursons, J., et al. 2015. Cell Commun Signal. 13: 26. PMID: 25975820
- Resveratrol Alleviates Cadmium-Induced Damage and Overexpression of Epidermal Growth Factor Receptor and its Downstream Signaling Proteins in the Reproductive System of Male Swiss Albino Mice. | Mitra, S., et al. 2016. J Environ Pathol Toxicol Oncol. 35: 73-90. PMID: 27279585
- TGF-β1/PTEN/PI3K signaling plays a critical role in the anti-proliferation effect of tetrandrine in human colon cancer cells. | Chen, QZ., et al. 2017. Int J Oncol. 50: 1011-1021. PMID: 28197642
- In vitro Cytotoxic Activities of the Oral Platinum(IV) Prodrug Oxoplatin and HSP90 Inhibitor Ganetespib against a Panel of Gastric Cancer Cell Lines. | Klameth, L., et al. 2017. J Cancer. 8: 1733-1743. PMID: 28819369
- PPP2R1A regulated by PAX3/FOXO1 fusion contributes to the acquisition of aggressive behavior in PAX3/FOXO1-positive alveolar rhabdomyosarcoma. | Akaike, K., et al. 2018. Oncotarget. 9: 25206-25215. PMID: 29861864
- Uncovering the anti-proliferation mechanism and bioactive compounds in red kidney bean coat against B16-F10 melanoma cells by metabolomics and network pharmacology analysis. | Nie, JH., et al. 2019. Food Funct. 10: 912-924. PMID: 30698192
- Non-integrin laminin receptor (LamR) plays a role in axonal outgrowth from chicken DRG via modulating the Akt and Erk signaling. | Mrówczyńska, E., et al. 2024. Front Cell Dev Biol. 12: 1433947. PMID: 39144252
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-Akt1/2/3 Antibody (A-12) | sc-271964 | 200 µg/ml | $322.00 | |||
p-Akt1/2/3 Antibody (A-12): m-IgGκ BP-HRP Bundle | sc-552325 | 200 µg Ab; 40 µg BP | $361.00 | |||
p-Akt1/2/3 (A-12) Neutralizing Peptide | sc-271964 P | 100 µg/0.5 ml | $69.00 |