Nup88 CRISPR/Cas9 KO Plasmid (m): sc-422392
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nup88 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Nup88 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nup88 Antibody (H-7): sc-365868
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nup88 CRISPR/Cas9 KO Plasmid (m) | sc-422392 | 20 µg | $397.00 |
Overview
Nup88 (nucleoporin 88 kDa) is an essential component of the nuclear pore complex that contributes to nucleocytoplasmic transport and overall nuclear envelope organization. By associating with other nucleoporins and transport receptors, Nup88 helps regulate the trafficking of proteins and RNAs that control transcriptional programs, cell cycle progression, and stress-responsive signaling. Altered Nup88 abundance or localization has been linked to defects in nuclear transport fidelity and genome maintenance, processes frequently perturbed in proliferative and neurodevelopmental contexts. Mouse Nup88 is therefore widely used to interrogate nuclear pore–dependent regulation of signaling and gene expression in normal physiology and disease-relevant cellular states.
Nup88 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nup88 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nup88 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nup88 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Nup88 protein expression.
This CRISPR knockout system enables efficient generation of Nup88-deficient cell models for investigation of Nup88 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nup88 exon(s) critical for Nup88 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nup88 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Nup88 CRISPR/Cas9 KO Plasmid (m) and Nup88 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nup88 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Nup88 HDR Plasmid (m) and Nup88 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nup88 homology arms to support homology-directed repair at defined Nup88 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.