Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NPR-C CRISPR/Cas9 KO Plasmid (m): sc-421949
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NPR-C CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NPR-C genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NPR-C Antibody (E-5): sc-515449
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NPR-C CRISPR/Cas9 KO Plasmid (m) | sc-421949 | 20 µg | $397.00 |
Overview
Mouse Npr3 encodes natriuretic peptide receptor C (NPR-C), a membrane receptor that binds ANP, BNP, and CNP to regulate their availability and downstream signaling. NPR-C is widely characterized as a clearance receptor that modulates natriuretic peptide-driven cGMP signaling by controlling ligand turnover, thereby influencing vascular tone, cardiac remodeling, renal sodium handling, and fluid homeostasis. Beyond peptide clearance, NPR-C has been linked to Gi/adenylyl cyclase-associated signaling and receptor-mediated endocytosis in multiple cell types. Dysregulation of NPR-C–mediated pathways has been associated with cardiometabolic and cardiovascular phenotypes, making Npr3 a useful target for mechanistic studies of blood pressure regulation and tissue remodeling.
NPR-C CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Npr3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Npr3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Npr3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NPR-C protein expression.
This CRISPR knockout system enables efficient generation of Npr3-deficient cell models for investigation of NPR-C signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Npr3 exon(s) critical for NPR-C function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Npr3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NPR-C CRISPR/Cas9 KO Plasmid (m) and NPR-C CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Npr3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NPR-C HDR Plasmid (m) and NPR-C HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Npr3 homology arms to support homology-directed repair at defined Npr3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.