Norpeg CRISPR/Cas9 KO Plasmid (h): sc-408852
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Norpeg CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Norpeg genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Norpeg CRISPR/Cas9 KO Plasmid (h) | sc-408852 | 20 µg | $397.00 |
Overview
RAI14 encodes Norpeg, a cytoskeletal-associated protein implicated in organizing actin-rich structures and regulating cell morphology, adhesion, and motility. Norpeg has been linked to signaling processes that coordinate cytoskeletal remodeling, supporting roles in cell migration and epithelial/mesenchymal dynamics. Expression and functional studies have associated RAI14 with contexts of altered cellular growth control and tissue architecture, making it relevant to investigations of proliferative and invasive phenotypes. As a scaffold-like factor, Norpeg provides a useful entry point for dissecting how cytoskeletal organization intersects with intracellular signaling and stress-responsive programs.
Norpeg CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RAI14 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RAI14 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RAI14 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Norpeg protein expression.
This CRISPR knockout system enables efficient generation of RAI14-deficient cell models for investigation of Norpeg signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RAI14 exon(s) critical for Norpeg function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RAI14 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Norpeg CRISPR/Cas9 KO Plasmid (h) and Norpeg CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RAI14 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Norpeg HDR Plasmid (h) and Norpeg HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RAI14 homology arms to support homology-directed repair at defined RAI14 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.