neuropilin-1 CRISPR/Cas9 KO Plasmid (m): sc-421964
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- neuropilin-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the neuropilin-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: neuropilin-1 Antibody (A-12): sc-5307
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
neuropilin-1 CRISPR/Cas9 KO Plasmid (m) | sc-421964 | 20 µg | $397.00 |
Overview
Mouse Nrp1 encodes neuropilin-1, a multifunctional transmembrane co-receptor that binds class 3 semaphorins and VEGF family ligands to modulate axon guidance, angiogenic sprouting, and endothelial tip cell behavior. Neuropilin-1 coordinates signaling with VEGFR2, plexins, and integrins, influencing cytoskeletal dynamics, cell migration, and vascular permeability through pathways including PI3K/AKT, MAPK/ERK, and Rho GTPase networks. In immune and stromal compartments, NRP1 contributes to cell trafficking and tissue remodeling, linking it to inflammatory microenvironments and developmental processes. Dysregulated NRP1 activity has been associated with aberrant vascular patterning and tumor-associated angiogenesis, making it a frequently studied node in neurovascular and microenvironmental biology.
neuropilin-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nrp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nrp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nrp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish neuropilin-1 protein expression.
This CRISPR knockout system enables efficient generation of Nrp1-deficient cell models for investigation of neuropilin-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nrp1 exon(s) critical for neuropilin-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nrp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by neuropilin-1 CRISPR/Cas9 KO Plasmid (m) and neuropilin-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nrp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by neuropilin-1 HDR Plasmid (m) and neuropilin-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nrp1 homology arms to support homology-directed repair at defined Nrp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.