Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Myosin Id CRISPR/Cas9 KO Plasmid (h): sc-403425
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Myosin Id CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Myosin Id genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Myosin Id Antibody (H-1): sc-515292
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Myosin Id CRISPR/Cas9 KO Plasmid (h) | sc-403425 | 20 µg | $397.00 |
Overview
MYO1D encodes Myosin Id, an actin-based, unconventional myosin implicated in organizing the cortical cytoskeleton and regulating membrane dynamics. Through interactions with actin filaments and membrane-associated complexes, MYO1D contributes to cell polarity, vesicle trafficking, and motility-related processes that influence tissue architecture. Altered MYO1D expression or function has been associated with dysregulated cytoskeletal remodeling and signaling networks linked to epithelial organization, migration, and tumor biology in several contexts. As a mechanochemical effector at the cell periphery, MYO1D is studied for its roles in coupling actin dynamics to membrane tension and directional transport.
Myosin Id CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MYO1D gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MYO1D together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MYO1D open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Myosin Id protein expression.
This CRISPR knockout system enables efficient generation of MYO1D-deficient cell models for investigation of Myosin Id signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MYO1D exon(s) critical for Myosin Id function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MYO1D genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Myosin Id CRISPR/Cas9 KO Plasmid (h) and Myosin Id CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MYO1D locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Myosin Id HDR Plasmid (h) and Myosin Id HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MYO1D homology arms to support homology-directed repair at defined MYO1D target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.