Mucin 4/MUC4 CRISPR/Cas9 KO Plasmid (m): sc-431204
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Mucin 4/MUC4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Mucin 4/MUC4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Mucin 4/MUC4 Antibody (1G8): sc-33654
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Mucin 4/MUC4 CRISPR/Cas9 KO Plasmid (m) | sc-431204 | 20 µg | $397.00 |
Overview
Muc4 encodes mucin 4 (MUC4), a high–molecular weight, heavily O-glycosylated transmembrane mucin that contributes to epithelial surface protection, hydration, and lubrication. Through its extended extracellular domain and cell-surface localization, MUC4 influences cell–cell and cell–matrix interactions, modulates barrier properties, and can affect receptor-proximal signaling and downstream pathways linked to proliferation, adhesion, and migration. In mouse tissues, Muc4 expression is associated with mucosal epithelia and secretory programs, integrating with glycosylation and membrane trafficking processes that shape the glycocalyx. Dysregulated mucin expression and altered glycosylation are frequently studied in the context of inflammation, epithelial remodeling, and tumor-associated changes in adhesion and signaling.
Mucin 4/MUC4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Muc4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Muc4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Muc4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Mucin 4/MUC4 protein expression.
This CRISPR knockout system enables efficient generation of Muc4-deficient cell models for investigation of Mucin 4/MUC4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Muc4 exon(s) critical for Mucin 4/MUC4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Muc4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Mucin 4/MUC4 CRISPR/Cas9 KO Plasmid (m) and Mucin 4/MUC4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Muc4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Mucin 4/MUC4 HDR Plasmid (m) and Mucin 4/MUC4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Muc4 homology arms to support homology-directed repair at defined Muc4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.