Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MAGE-A1 CRISPR/Cas9 KO Plasmid (h): sc-401056
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MAGE-A1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MAGE-A1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MAGE-A1 Antibody (MA454): sc-20033
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MAGE-A1 CRISPR/Cas9 KO Plasmid (h) | sc-401056 | 20 µg | $397.00 |
Overview
MAGEA1 encodes MAGE-A1, a cancer-testis antigen normally restricted to germ cells but frequently re-expressed in diverse malignancies, where it can contribute to tumor-associated transcriptional and proteostasis programs. MAGE-A1 belongs to the type I MAGE family and is implicated in protein–protein interactions that modulate ubiquitin-dependent pathways, including regulation of E3 ligase activity and turnover of signaling components. Aberrant MAGEA1 expression is associated with altered cell-cycle control, stress responses, and immune-recognition phenotypes that shape tumor biology. As a lineage-restricted antigen, MAGE-A1 is also widely used as a molecular marker in studies of tumor immunogenicity and epigenetic derepression.
MAGE-A1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MAGEA1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MAGEA1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MAGEA1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MAGE-A1 protein expression.
This CRISPR knockout system enables efficient generation of MAGEA1-deficient cell models for investigation of MAGE-A1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MAGEA1 exon(s) critical for MAGE-A1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MAGEA1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MAGE-A1 CRISPR/Cas9 KO Plasmid (h) and MAGE-A1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MAGEA1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MAGE-A1 HDR Plasmid (h) and MAGE-A1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MAGEA1 homology arms to support homology-directed repair at defined MAGEA1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.