Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
LZTR1 CRISPR/Cas9 KO Plasmid (h): sc-404531
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LZTR1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LZTR1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LZTR1 Antibody (E-12): sc-390166
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LZTR1 CRISPR/Cas9 KO Plasmid (h) | sc-404531 | 20 µg | $397.00 |
Overview
LZTR1 (leucine zipper–like transcription regulator 1) encodes a BTB-Kelch family protein that localizes predominantly to the Golgi and functions as a substrate adaptor for CUL3-based E3 ubiquitin ligase complexes. Through ubiquitin-dependent control of protein stability, LZTR1 modulates RAS/MAPK pathway signaling by regulating the turnover and spatial organization of RAS family GTPases and related signaling components. This gene is linked to cellular processes including signal transduction, proteostasis, and vesicular trafficking, with downstream effects on proliferation and differentiation programs. Genetic alterations in LZTR1 have been associated with RASopathy-related phenotypes and tumor susceptibility contexts, supporting its relevance for mechanistic studies of aberrant RAS signaling.
LZTR1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the LZTR1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the LZTR1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the LZTR1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LZTR1 protein expression.
This CRISPR knockout system enables efficient generation of LZTR1-deficient cell models for investigation of LZTR1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting LZTR1 exon(s) critical for LZTR1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple LZTR1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LZTR1 CRISPR/Cas9 KO Plasmid (h) and LZTR1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the LZTR1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LZTR1 HDR Plasmid (h) and LZTR1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by LZTR1 homology arms to support homology-directed repair at defined LZTR1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.