Laminin α-3 CRISPR/Cas9 KO Plasmid (m): sc-421376
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Laminin α-3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Laminin α-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Laminin α-3 CRISPR/Cas9 KO Plasmid (m) | sc-421376 | 20 µg | $397.00 |
Overview
Lama3 encodes laminin α-3, a key subunit of laminin-332 that integrates into basement membranes to support epithelial cell adhesion, polarity, and directed migration. By binding integrins and collaborating with hemidesmosome components, laminin α-3 helps coordinate focal adhesion dynamics and cytoskeletal organization through adhesion-linked signaling pathways such as FAK/SRC and PI3K–AKT. In mouse tissues, Lama3 contributes to epidermal–dermal anchoring and epithelial barrier integrity, and its dysregulation is commonly studied in contexts of impaired wound re-epithelialization and altered epithelial–stromal interactions. Experimental models targeting Lama3 are used to interrogate extracellular matrix assembly, cell–matrix mechanotransduction, and basement membrane remodeling relevant to tissue homeostasis and disease-associated microenvironments.
Laminin α-3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Lama3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Lama3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Lama3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Laminin α-3 protein expression.
This CRISPR knockout system enables efficient generation of Lama3-deficient cell models for investigation of Laminin α-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Lama3 exon(s) critical for Laminin α-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Lama3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Laminin α-3 CRISPR/Cas9 KO Plasmid (m) and Laminin α-3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Lama3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Laminin α-3 HDR Plasmid (m) and Laminin α-3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Lama3 homology arms to support homology-directed repair at defined Lama3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.