Ki67 CRISPR/Cas9 KO Plasmid (m): sc-421656
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ki67 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ki67 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ki67 CRISPR/Cas9 KO Plasmid (m) | sc-421656 | 20 µg | $397.00 |
Overview
Mki67 encodes the proliferation marker protein Ki67, a nuclear factor tightly linked to cell-cycle progression and broadly expressed in actively cycling cells while absent in quiescence. Ki67 associates with chromatin and the mitotic chromosome periphery, supporting chromosome organization and contributing to proper mitotic progression and nucleolar structure during interphase. Its expression integrates with core cell-cycle control programs, including pathways governing DNA replication, mitosis, and checkpoint regulation, making it widely used to quantify proliferative states in tissues and cultured cells. Dysregulated Ki67 levels are frequently correlated with hyperproliferative phenotypes and are leveraged in studies of tumor biology, tissue regeneration, and immune cell expansion in mouse models.
Ki67 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Mki67 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Mki67 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Mki67 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ki67 protein expression.
This CRISPR knockout system enables efficient generation of Mki67-deficient cell models for investigation of Ki67 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Mki67 exon(s) critical for Ki67 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Mki67 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ki67 CRISPR/Cas9 KO Plasmid (m) and Ki67 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Mki67 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ki67 HDR Plasmid (m) and Ki67 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Mki67 homology arms to support homology-directed repair at defined Mki67 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.