KDEL receptor 1 CRISPR/Cas9 KO Plasmid (m): sc-426938
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KDEL receptor 1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KDEL receptor 1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KDEL receptor 1 CRISPR/Cas9 KO Plasmid (m) | sc-426938 | 20 µg | $397.00 |
Overview
Kdelr1 encodes KDEL receptor 1, a seven-transmembrane sorting receptor that recognizes C-terminal KDEL-like retrieval motifs on soluble ER-resident proteins and mediates their retrograde transport from the Golgi to the ER. By cycling between the ER and Golgi in a pH-dependent manner, KDEL receptor 1 helps maintain ER proteostasis, supports proper folding capacity, and preserves secretory pathway fidelity. Disruption of ER–Golgi trafficking and ER quality control can trigger unfolded protein response signaling and altered secretion, processes broadly implicated in stress sensitivity and degeneration-associated phenotypes in experimental systems. In mouse models, Kdelr1 perturbation is therefore relevant for dissecting secretory pathway homeostasis and cellular stress responses across immune, neuronal, and metabolically active tissues.
KDEL receptor 1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Kdelr1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Kdelr1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Kdelr1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KDEL receptor 1 protein expression.
This CRISPR knockout system enables efficient generation of Kdelr1-deficient cell models for investigation of KDEL receptor 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Kdelr1 exon(s) critical for KDEL receptor 1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Kdelr1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KDEL receptor 1 CRISPR/Cas9 KO Plasmid (m) and KDEL receptor 1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Kdelr1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KDEL receptor 1 HDR Plasmid (m) and KDEL receptor 1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Kdelr1 homology arms to support homology-directed repair at defined Kdelr1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.