Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Kaiso CRISPR/Cas9 KO Plasmid (h): sc-417904
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Kaiso CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Kaiso genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Kaiso Antibody (D-10): sc-365428
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Kaiso CRISPR/Cas9 KO Plasmid (h) | sc-417904 | 20 µg | $397.00 |
Overview
ZBTB33 encodes Kaiso, a BTB/POZ domain zinc-finger transcription factor that binds sequence-specific Kaiso binding sites and methylated CpG dinucleotides to modulate gene expression programs. Through interactions with corepressor complexes and catenin family signaling components, Kaiso helps coordinate chromatin-dependent transcriptional repression and context-dependent transcriptional switching. Kaiso-regulated networks intersect with pathways controlling epithelial differentiation, cell–cell adhesion, and Wnt/β-catenin-associated transcriptional outputs. Dysregulated ZBTB33 activity or altered Kaiso occupancy has been studied in cancer biology, where epigenetic state and transcriptional rewiring contribute to invasive phenotypes and tumor progression.
Kaiso CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ZBTB33 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ZBTB33 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ZBTB33 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Kaiso protein expression.
This CRISPR knockout system enables efficient generation of ZBTB33-deficient cell models for investigation of Kaiso signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ZBTB33 exon(s) critical for Kaiso function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ZBTB33 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Kaiso CRISPR/Cas9 KO Plasmid (h) and Kaiso CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ZBTB33 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Kaiso HDR Plasmid (h) and Kaiso HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ZBTB33 homology arms to support homology-directed repair at defined ZBTB33 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.