IL-12Rβ2 CRISPR/Cas9 KO Plasmid (h): sc-406442
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-12Rβ2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IL-12Rβ2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-12Rβ2 Antibody (2H6): sc-293379
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-12Rβ2 CRISPR/Cas9 KO Plasmid (h) | sc-406442 | 20 µg | $397.00 |
Overview
IL12RB2 encodes the interleukin-12 receptor beta 2 chain (IL-12Rβ2), a signaling subunit that pairs with IL-12Rβ1 to form the functional IL-12 receptor complex on T cells and NK cells. Upon IL-12 engagement, the receptor activates JAK2/TYK2-dependent STAT4 signaling, promoting Th1 polarization, IFN-γ production, and cytotoxic immune programs that shape cell-mediated immunity. IL-12Rβ2 expression and signaling competence influence differentiation and effector functions within inflammatory and tumor-associated immune microenvironments, linking this axis to immune dysregulation and altered anti-pathogen or anti-tumor responses. Dysregulated IL12RB2 activity has been studied in contexts of chronic inflammation, immune evasion, and susceptibility to infection through impaired STAT4-driven transcriptional networks.
IL-12Rβ2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IL12RB2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IL12RB2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IL12RB2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IL-12Rβ2 protein expression.
This CRISPR knockout system enables efficient generation of IL12RB2-deficient cell models for investigation of IL-12Rβ2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IL12RB2 exon(s) critical for IL-12Rβ2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IL12RB2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IL-12Rβ2 CRISPR/Cas9 KO Plasmid (h) and IL-12Rβ2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IL12RB2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IL-12Rβ2 HDR Plasmid (h) and IL-12Rβ2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IL12RB2 homology arms to support homology-directed repair at defined IL12RB2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.