Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HSPA8/HSC70 CRISPR/Cas9 KO Plasmid (h): sc-418508
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSPA8/HSC70 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HSPA8/HSC70 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSPA8/HSC70 Antibody (B-6): sc-7298
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSPA8/HSC70 CRISPR/Cas9 KO Plasmid (h) | sc-418508 | 20 µg | $397.00 |
Overview
HSPA8 (HSC70) is a constitutively expressed HSP70-family molecular chaperone that supports proteostasis by promoting ATP-dependent folding, refolding, and triage of nascent or stress-damaged proteins. It participates in protein quality control pathways including chaperone-mediated autophagy via lysosomal LAMP2A, endocytosis and clathrin uncoating, and regulation of stress granule dynamics, thereby influencing cellular responses to proteotoxic and metabolic stress. Through interactions with co-chaperones and the ubiquitin–proteasome system, HSPA8 helps govern protein turnover, organelle homeostasis, and signaling robustness across diverse cell types. Dysregulation of HSPA8-linked chaperone networks has been associated with protein aggregation biology and altered stress-adaptation programs observed in neurodegenerative and cancer-related research contexts.
HSPA8/HSC70 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HSPA8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HSPA8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HSPA8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HSPA8/HSC70 protein expression.
This CRISPR knockout system enables efficient generation of HSPA8-deficient cell models for investigation of HSPA8/HSC70 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HSPA8 exon(s) critical for HSPA8/HSC70 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HSPA8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HSPA8/HSC70 CRISPR/Cas9 KO Plasmid (h) and HSPA8/HSC70 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HSPA8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HSPA8/HSC70 HDR Plasmid (h) and HSPA8/HSC70 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HSPA8 homology arms to support homology-directed repair at defined HSPA8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.