HMG-3/HMGB3 CRISPR/Cas9 KO Plasmid (m): sc-420875
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HMG-3/HMGB3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HMG-3/HMGB3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HMG-3/HMGB3 CRISPR/Cas9 KO Plasmid (m) | sc-420875 | 20 µg | $397.00 |
Overview
Hmgb3 encodes the high mobility group box protein HMGB3 (HMG-3), a non-histone chromatin-associated factor that binds and bends DNA to modulate nucleosome architecture and genome accessibility. HMGB3 participates in the regulation of transcriptional programs, DNA replication and repair, and higher-order chromatin dynamics, thereby influencing cell-cycle progression and lineage-specific differentiation. Through its roles in chromatin remodeling and DNA damage–responsive processes, altered HMGB3 activity has been linked to dysregulated proliferation and aberrant hematopoietic and developmental gene expression networks. In mouse systems, Hmgb3 is frequently studied to dissect epigenetic control of stem/progenitor states and the molecular consequences of chromatin perturbation.
HMG-3/HMGB3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Hmgb3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Hmgb3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Hmgb3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HMG-3/HMGB3 protein expression.
This CRISPR knockout system enables efficient generation of Hmgb3-deficient cell models for investigation of HMG-3/HMGB3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Hmgb3 exon(s) critical for HMG-3/HMGB3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Hmgb3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HMG-3/HMGB3 CRISPR/Cas9 KO Plasmid (m) and HMG-3/HMGB3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Hmgb3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HMG-3/HMGB3 HDR Plasmid (m) and HMG-3/HMGB3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Hmgb3 homology arms to support homology-directed repair at defined Hmgb3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.