Glutathione Peroxidase 2/GPX2 CRISPR/Cas9 KO Plasmid (h): sc-406827

Overview

GPX2 encodes glutathione peroxidase 2, a selenium-dependent antioxidant enzyme that reduces hydrogen peroxide and lipid hydroperoxides using glutathione as an electron donor, thereby limiting oxidative damage and preserving redox homeostasis. GPX2 activity interfaces with cellular peroxide detoxification and glutathione metabolism, shaping ROS-dependent signaling and protecting membranes from lipid peroxidation. In human tissues, GPX2 is prominently associated with epithelial barrier biology and stress responses, where altered expression has been linked to inflammation-driven oxidative stress and dysregulated proliferation programs. Perturbation of GPX2 can therefore impact pathways controlling redox balance, cell survival under oxidative challenge, and context-dependent transcriptional responses to ROS.

Glutathione Peroxidase 2/GPX2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GPX2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GPX2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GPX2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Glutathione Peroxidase 2/GPX2 protein expression.

This CRISPR knockout system enables efficient generation of GPX2-deficient cell models for investigation of Glutathione Peroxidase 2/GPX2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting GPX2 exon(s) critical for Glutathione Peroxidase 2/GPX2 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple GPX2 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Glutathione Peroxidase 2/GPX2 CRISPR/Cas9 KO Plasmid (h) and Glutathione Peroxidase 2/GPX2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GPX2 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Glutathione Peroxidase 2/GPX2 HDR Plasmid (h) and Glutathione Peroxidase 2/GPX2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GPX2 homology arms to support homology-directed repair at defined GPX2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.