Glucose Transporter Glut8 CRISPR/Cas9 KO Plasmid (m): sc-424976
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Glucose Transporter Glut8 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Glucose Transporter Glut8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Glucose Transporter Glut8 CRISPR/Cas9 KO Plasmid (m) | sc-424976 | 20 µg | $397.00 |
Overview
Slc2a8 encodes glucose transporter GLUT8, a facilitative hexose transporter that contributes to intracellular and membrane-associated glucose handling in mouse cells. GLUT8 supports cellular energy balance by regulating glucose availability for glycolysis and downstream metabolic pathways, with functional links to insulin-responsive nutrient sensing and organ-specific metabolic homeostasis. In specialized tissues, altered Slc2a8 activity can influence glucose flux, oxidative metabolism, and stress responses that intersect with endocrine and reproductive physiology. Dysregulation of GLUT family transport processes is broadly relevant to models of metabolic dysfunction and to studies of how nutrient transport shapes cellular growth, differentiation, and survival programs.
Glucose Transporter Glut8 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Slc2a8 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Slc2a8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Slc2a8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Glucose Transporter Glut8 protein expression.
This CRISPR knockout system enables efficient generation of Slc2a8-deficient cell models for investigation of Glucose Transporter Glut8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Slc2a8 exon(s) critical for Glucose Transporter Glut8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Slc2a8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Glucose Transporter Glut8 CRISPR/Cas9 KO Plasmid (m) and Glucose Transporter Glut8 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Slc2a8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Glucose Transporter Glut8 HDR Plasmid (m) and Glucose Transporter Glut8 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Slc2a8 homology arms to support homology-directed repair at defined Slc2a8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.