m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for GDF-15 Antibody (H-2) for WB applications. This reagent is now offered in a bundle with GDF-15 Antibody (H-2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Growth differentiation factor 15 (GDF-15), also known as PDF, MIC-1, PLAB, NAG-1 or PTGF-β, is a member of the transforming growth factor beta (TGF-beta) superfamily. Synthesized intracellularly, the protein is secreted as a dimer linked by disulfide bonds. Epithelial cells and macrophages are the sites of strongest GDF-15 expression, although it is widely expressed in adult tissue. In the brain, GDF-15 expression occurs in the choroid plexus, from which the protein is secreted into the cerebrospinal fluid. The gene for GDF-15 is responsive to p53 tumor suppressor protein, and in cultured cerebellar granule neurons GDF-15 can prevent cell death by the activation of Akt and inhibition of ERK. GDF-15 acts as a trophic factor for certain classes of neurons, promoting cell survival and differentiation. Overexpression of GDF-15 occurs in prostate cancer, and may be a means of diagnosis. In the uterus, GDF-15 may work to suppress maternally derived proinflammatory cytokines, thereby promoting fetal survival.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Please tell me the blocking peptide corresponding to this antibody (sc-515675).
Thank you for your question. We do not offer a blocking peptide for this antibody.
回答者: Tech Support Europe
I have a question. is western blot data non-reducing conditions or reducing conditions?
I did WB to detect gdf15 in LNCaP by non-reducing conditions. I got 2 band : one above 40 kDa, the other around 35 kDa.
Thank you for your question. Our Western blot data with this antibody is under reducing conditions. We have not tested the antibody under non-reducing conditions.
回答者: Technical Support
I have a rat cell line, can this ab detect GDF15 from rat?
Thank you for your question. Yes, this antibody is recommended for detection of GDF15 of mouse, rat and human origin. We have Western blot data on our website in the rat cell line, RIN-m5F.
回答者: Technical Support
Has anyone any information that this Ab can detect human GDF15. According to the protein alignments in aa 41-62 the Human sequence differs by an additional 7 amino acids. Would be keen to see some experimental data if anyone has it. Thanks
質問者: Satish Patel
Thank you for your question. Yes, we have human cell data on this antibody in LNCaP cells on the product page:
If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
回答者: Tech Service
I need to use an antibody to detect the protein in samples of avian origin. Do you know if sc-515675: GDF-15 (H-2) mouse monoclonal antibody will work?
質問者: Dr Ninau Qelp
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Works well on murine cardiac samples1h blocking in mPBST, GDF15 ab ON at 4°C, secondary ab 1 h at RT; Ab detected 2 bands: one slightly above 40 kDa, the other around 25 kDa