GDF-15抗体(H-2) mouse, rat と human 由来のprecursor and mature GDF-15 WB, IP, IF と ELISAでの検出にはお勧めします

GDF-15抗体(H-2): sc-515675

GDF-15抗体(H-2)は、 1件のレビューによって、 5つ星のうち 4.0と 評価されています。
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データシート

    • GDF-15抗体 (H-2)はマウスモノクローナルIgG2a (kappa light chain)です。200 µg/mlで提供
    • rat由来ののN-terminusの辺りのアミノ酸41-62の間に位置するエピトープに特異的
    • 反-GDF-15抗体 (H-2) mouse, rat と human 由来のprecursor and mature GDF-15 WB, IP, IF と ELISAでの検出にはお勧めします
    • 反-GDF-15抗体 (H-2) IP用のアガロース、に共役での利用可能です。WB、IHC(P)とELISA用のHRP、IF、IHC(P)とFCM用のphycoerythrin または FITC、に共役での利用可能です。
    • WB (RGB)、IF、IHC(P)、FCM、iBright™ FL1000、FluorChem™、Typhoon、Azureと他の同等システムでRGB蛍光イメージングシステム用のAlexa Fluor® 488Alexa Fluor® 546Alexa Fluor® 594 または Alexa Fluor® 647、に共役での利用可能です。
    • WB (NIR)、IF、FCMとLI-COR®/Odyssey®、iBright™ FL1000、FluorChem™、Typhoon、Azureと他の同等システムで近赤外(NIR)検出法用のAlexa Fluor® 680 または Alexa Fluor® 790、に共役での利用可能です。
    • GDF-15 (H-2): sc-515675無料の10 µgサンプルを受け取るには、ご連絡ください。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for GDF-15 Antibody (H-2) for WB applications. This reagent is now offered in a bundle with GDF-15 Antibody (H-2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Please tell me the blocking peptide corresponding to this antibody (sc-515675).

質問者: yuki13
Thank you for your question. We do not offer a blocking peptide for this antibody.
回答者: Tech Support Europe
投稿日: 2020-07-29

I have a question. is western blot data non-reducing conditions or reducing conditions? I did WB to detect gdf15 in LNCaP by non-reducing conditions. I got 2 band : one above 40 kDa, the other around 35 kDa.

質問者: Col12
Thank you for your question. Our Western blot data with this antibody is under reducing conditions. We have not tested the antibody under non-reducing conditions.
回答者: Technical Support
投稿日: 2019-05-25

I have a rat cell line, can this ab detect GDF15 from rat?

質問者: M76gmm
Thank you for your question. Yes, this antibody is recommended for detection of GDF15 of mouse, rat and human origin. We have Western blot data on our website in the rat cell line, RIN-m5F.
回答者: Technical Support
投稿日: 2019-01-31

Has anyone any information that this Ab can detect human GDF15. According to the protein alignments in aa 41-62 the Human sequence differs by an additional 7 amino acids. Would be keen to see some experimental data if anyone has it. Thanks

質問者: Satish Patel
Thank you for your question. Yes, we have human cell data on this antibody in LNCaP cells on the product page: https://www.scbt.com/scbt/product/gdf-15-antibody-h-2?requestFrom=search If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
回答者: Tech Service
投稿日: 2017-06-28

I need to use an antibody to detect the protein in samples of avian origin. Do you know if sc-515675: GDF-15 (H-2) mouse monoclonal antibody will work?

質問者: Dr Ninau Qelp
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
回答者: Technical Support
投稿日: 2017-03-06
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によって、 5つ星のうち 4 と評価されています。 Works well on murine cardiac samples 1h blocking in mPBST, GDF15 ab ON at 4°C, secondary ab 1 h at RT; Ab detected 2 bands: one slightly above 40 kDa, the other around 25 kDa
投稿日: 2017-11-27
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