FIH-1 Antibody (F-11): sc-365128

FIH-1 Antibody (F-11) is a mouse monoclonal IgG2b antibody that detects Factor inhibiting HIF-1 (FIH-1) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-FIH-1 antibody (F-11) plays a crucial role in studying the regulation of hypoxia-inducible factor 1 alpha (HIF-1α) by functioning as an asparaginyl hydroxylase, specifically catalyzing the hydroxylation of asparagine residue 803 within the carboxy-terminal transactivation domain of HIF-1α. This post-translational modification is significant as FIH-1 (F-11) antibody helps researchers understand how FIH-1 inhibits the interaction of HIF-1α with essential co-activators, thereby modulating the transcriptional activity of genes involved in the cellular response to hypoxia. Additionally, FIH-1 monoclonal antibody (F-11) enables research into FIH-1′s interaction with the von Hippel-Lindau (VHL) tumor suppressor protein, further contributing to the regulation of HIF-1α activity. Studies using FIH-1 (F-11) antibody have revealed FIH-1′s localization in the cytoplasm of transiently transfected human osteosarcoma cells, underscoring its functional importance in cellular signaling pathways. The structural characteristics of FIH-1, including its jellyroll-like β-barrel and ferrous-binding triad residues, are vital for its enzymatic activity and stability, making FIH-1 (F-11) monoclonal antibody an essential tool for researchers studying hypoxia-related processes and their implications in various diseases.

The preferred FIH-1 antibody is FIH-1 Antibody (A-5): sc-271780.