Dynactin p62 Antibody (H-12): sc-55604

Dynactin p62 Antibody (H-12) is a mouse monoclonal IgG2a antibody that detects Dynactin p62 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Dynactin p62 plays a crucial role in the functionality of the dynactin complex, which is essential for the proper functioning of the microtubule-based motor cytoplasmic dynein, a key player in intracellular transport. Dynactin p62 is primarily located in the cytoplasm, where Dynactin p62 exhibits a punctate distribution, and is also found in the nucleus at elevated levels, highlighting Dynactin p62′s importance in cellular organization and transport processes. The presence of Dynactin p62 in the nucleus suggests a potential regulatory role in gene expression or nuclear transport, making Dynactin p62 a significant focus for studies related to cellular dynamics and signaling pathways. Additionally, the p62 subunit contains a highly conserved cysteine-rich motif that facilitates interaction with the actin-related protein 1 (Arp1), further emphasizing Dynactin p62′s structural and functional importance in linking dynein and dynactin to the cortical cytoskeleton. With diverse applications and critical role in cellular processes, anti-Dynactin p62 antibody (H-12) serves as an invaluable tool for researchers investigating the mechanisms of intracellular transport and cytoskeletal dynamics.

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Dynactin p62 Antibody (H-12) References:

1. Cytoplasmic dynein and dynactin as likely candidates for microtubule-dependent apical targeting of pancreatic zymogen granules.  |  Kraemer, J., et al. 1999. Eur J Cell Biol. 78: 265-77. PMID: 10350215
2. Analysis of dynactin subcomplexes reveals a novel actin-related protein associated with the arp1 minifilament pointed end.  |  Eckley, DM., et al. 1999. J Cell Biol. 147: 307-20. PMID: 10525537
3. Interaction of the p62 subunit of dynactin with Arp1 and the cortical actin cytoskeleton.  |  Garces, JA., et al. Curr Biol. 9: 1497-500. PMID: 10607597
4. A dynactin subunit with a highly conserved cysteine-rich motif interacts directly with Arp1.  |  Karki, S., et al. 2000. J Biol Chem. 275: 4834-9. PMID: 10671518
5. Immunolocalization of cytoplasmic dynein and dynactin subunits in cultured macrophages: enrichment on early endocytic organelles.  |  Habermann, A., et al. 2001. J Cell Sci. 114: 229-240. PMID: 11112706
6. Copper-dependent interaction of dynactin subunit p62 with the N terminus of ATP7B but not ATP7A.  |  Lim, CM., et al. 2006. J Biol Chem. 281: 14006-14. PMID: 16554302
7. The p25 subunit of the dynactin complex is required for dynein-early endosome interaction.  |  Zhang, J., et al. 2011. J Cell Biol. 193: 1245-55. PMID: 21708978
8. Dynactin's pointed-end complex is a cargo-targeting module.  |  Yeh, TY., et al. 2012. Mol Biol Cell. 23: 3827-37. PMID: 22918948
9. Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit.  |  Yeh, TY., et al. 2013. EMBO J. 32: 1023-35. PMID: 23455152
10. A PIK3C3-ankyrin-B-dynactin pathway promotes axonal growth and multiorganelle transport.  |  Lorenzo, DN., et al. 2014. J Cell Biol. 207: 735-52. PMID: 25533844

The preferred Dynactin p62 antibody is Dynactin p62 Antibody (H-4): sc-55603.