DNA pol α Antibody (G-5): sc-365327
- DNA pol α Antibody (G-5) is a mouse monoclonal IgA κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 3-31 near the N-terminus of DNA pol α of human origin
- recommended for detection of DNA pol α of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgGκ BP-HRP is the preferred secondary detection reagent for DNA pol α Antibody (G-5) for WB applications. This reagent is now offered in a bundle with DNA pol α Antibody (G-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
QUICK LINKS
SEE ALSO...
DNA pol α Antibody (G-5) is a mouse monoclonal IgA antibody that detects DNA pol α in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DNA pol α plays a crucial role in DNA replication, particularly in the synthesis of the RNA-DNA primer necessary for initiating DNA strand synthesis, which is vital for maintaining genomic integrity. Anti-DNA pol α antibody (G-5) recognizes a multi-subunit enzyme composed of a catalytic subunit and several accessory proteins, which enhance stability and activity during the replication process. Proper functioning of DNA pol α is essential, as any deficiencies or malfunctions can result in replication errors, ultimately compromising cellular health and function. DNA pol α (G-5) monoclonal antibody serves as an invaluable tool for researchers studying the mechanisms of DNA replication and repair, as well as the implications of these processes in various diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DNA pol α Antibody (G-5) References:
- Aberrant DNA polymerase alpha is excluded from the nucleus by defective import and degradation in the nucleus. | Eichinger, CS., et al. 2009. J Biol Chem. 284: 30604-14. PMID: 19726690
- DNA Polymerase α Subunit Residues and Interactions Required for Efficient Initiation Complex Formation Identified by a Genetic Selection. | Lindow, JC., et al. 2015. J Biol Chem. 290: 16851-60. PMID: 25987558
- Activity and fidelity of human DNA polymerase α depend on primer structure. | Baranovskiy, AG., et al. 2018. J Biol Chem. 293: 6824-6843. PMID: 29555682
- DNA Polymerase alpha is essential for intracellular amplification of hepatitis B virus covalently closed circular DNA. | Tang, L., et al. 2019. PLoS Pathog. 15: e1007742. PMID: 31026293
- DNA polymerase α interacts with H3-H4 and facilitates the transfer of parental histones to lagging strands. | Li, Z., et al. 2020. Sci Adv. 6: eabb5820. PMID: 32923642
- A Mutation in DNA Polymerase α Rescues WEE1KO Sensitivity to HU. | Eekhout, T., et al. 2021. Int J Mol Sci. 22: PMID: 34502313
- Checkpoint kinase interaction with DNA polymerase alpha regulates replication progression during stress. | Hadjicharalambous, A., et al. 2023. Wellcome Open Res. 8: 327. PMID: 37766847
- Flexibility and Distributive Synthesis Regulate RNA Priming and Handoff in Human DNA Polymerase α-Primase. | Cordoba, JJ., et al. 2023. J Mol Biol. 435: 168330. PMID: 37884206
- A mechanistic model of primer synthesis from catalytic structures of DNA polymerase α-primase. | Mullins, EA., et al. 2024. Nat Struct Mol Biol. 31: 777-790. PMID: 38491139
- DNA polymerase alpha cofactors C1C2 function as primer recognition proteins. | Pritchard, CG., et al. 1983. J Biol Chem. 258: 9810-9. PMID: 6224785
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DNA pol α Antibody (G-5) | sc-365327 | 200 µg/ml | $322.00 | |||
DNA pol α Antibody (G-5): m-IgGκ BP-HRP Bundle | sc-552008 | 200 µg Ab; 40 µg BP | $361.00 | |||
DNA pol α (G-5) Neutralizing Peptide | sc-365327 P | 100 µg/0.5 ml | $69.00 |