Digoxigenin Antibody (21H8): sc-57583

Digoxigenin Antibody (21H8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Digoxigenin in non-species reactive applications such as western blotting (WB), immunofluorescence (IF), and immunohistochemistry. Anti-Digoxigenin antibody (21H8) is available as a non-conjugated format, providing researchers with a versatile tool for various experimental setups. Digoxigenin, a cardenolide steroid hapten, is uniquely found in the flowers and leaves of Digitalis purpurea and Digitalis lanata, and is derived from the hydrolysis of Digoxin. With a molecular weight of 390.513 g/mol, Digoxigenin plays a crucial role in increasing intracellular sodium levels, which can be particularly beneficial in the context of heart failure. Additionally, Digoxigenin′s ability to serve as a molecular probe for detecting both DNA and RNA makes Digoxigenin invaluable in molecular biology research. Notably, Digoxigenin can be easily conjugated to the 5′ or 3′ ends of oligonucleotides through chemical modifications, enhancing Digoxigenin′s utility across a wide range of applications, including gene expression studies and nucleic acid detection assays.

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Digoxigenin Antibody (21H8) References:

1. Detection of oligodendrocytes in tissue sections using PCR synthesis of digoxigenin-labeled probes.  |  Jalabi, W., et al. 2003. J Histochem Cytochem. 51: 913-9. PMID: 12810841
2. Nonradioactive PCR sequencing using digoxigenin.  |  Kösel, S., et al. 2003. Methods Mol Biol. 226: 347-54. PMID: 12958520
3. Dynamic force spectroscopy of the digoxigenin-antibody complex.  |  Neuert, G., et al. 2006. FEBS Lett. 580: 505-9. PMID: 16388805
4. Production of anti-digoxigenin antibody HRP conjugate for PCR-ELISA DIG detection system.  |  Gill, P., et al. 2006. J Immunoassay Immunochem. 27: 303-18. PMID: 16981644
5. Digoxigenin.  |  Green, MR. and Sambrook, J. 2022. Cold Spring Harb Protoc. 2022: PMID: 34907077
6. Quantification of digoxin by enzyme immunoassay: synthesis of a maleimide derivative of digoxigenin succinate for enzyme coupling.  |  Monji, N., et al. 1980. Experientia. 36: 1141-3. PMID: 6774880
7. Digoxigenin biotransformation.  |  Gault, H., et al. 1982. Clin Pharmacol Ther. 31: 695-704. PMID: 7075117
8. Digoxigenin-ampicillin conjugate for detection of penicillin-binding proteins by chemiluminescence.  |  Weigel, LM., et al. 1994. Antimicrob Agents Chemother. 38: 330-6. PMID: 8192459
9. Digoxigenin labeling.  |  McCreery, T. 1997. Mol Biotechnol. 7: 121-4. PMID: 9219226
10. Digoxigenin-labeled peptides for the immunological quantification of intracellular signaling proteins: application to the MAP kinase kinase isoform MEK2.  |  Blais, C., et al. 1997. Biotechniques. 23: 1098-103. PMID: 9421643
11. Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for In situ hybridization using CARD amplification.  |  Hopman, AH., et al. 1998. J Histochem Cytochem. 46: 771-7. PMID: 9603790
12. Post-rest contraction amplitude in myocytes from failing human ventricle.  |  Davia, K. and Harding, SE. 1998. Basic Res Cardiol. 93 Suppl 1: 33-7. PMID: 9833128

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Digoxigenin Antibody (21H8) has 1 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Digoxigenin Antibody (21H8) Specifications

Alternate Product Name: Digoxigenin

Product Clone Name: 21H8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Digoxigenin

Epitope Species:

Product Reactivity/Detection: free and bound Digoxigenin

Species Reactivity/Detection: NSR

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF, IHC(P)

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Digoxigenin Antibody (21H8): N/A