DDX32 CRISPR/Cas9 KO Plasmid (m): sc-430371
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DDX32 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DDX32 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DDX32 CRISPR/Cas9 KO Plasmid (m) | sc-430371 | 20 µg | $397.00 |
Overview
Dhx32 encodes the mouse DDX32 protein, a putative ATP-dependent RNA helicase of the DEAD-box family implicated in ATP-driven remodeling of RNA secondary structure and ribonucleoprotein complexes. Proteins in this class commonly regulate core RNA metabolic processes, including pre-mRNA splicing, RNA transport, ribosome biogenesis, and translation, linking DDX32 activity to control of gene expression programs. Altered RNA helicase function can perturb cell-cycle regulation, differentiation, and stress-adaptive transcriptional responses, making Dhx32 a useful locus for studying RNA processing pathways in development and disease-relevant cellular phenotypes. In mouse systems, Dhx32 interrogation supports mechanistic studies connecting RNA metabolism to signaling networks and genotype–phenotype relationships.
DDX32 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Dhx32 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Dhx32 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Dhx32 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DDX32 protein expression.
This CRISPR knockout system enables efficient generation of Dhx32-deficient cell models for investigation of DDX32 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Dhx32 exon(s) critical for DDX32 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Dhx32 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DDX32 CRISPR/Cas9 KO Plasmid (m) and DDX32 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Dhx32 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DDX32 HDR Plasmid (m) and DDX32 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Dhx32 homology arms to support homology-directed repair at defined Dhx32 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.