Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DAP-5 CRISPR/Cas9 KO Plasmid (m): sc-420151
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DAP-5 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DAP-5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DAP-5 Antibody (F-2): sc-137011
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAP-5 CRISPR/Cas9 KO Plasmid (m) | sc-420151 | 20 µg | $397.00 |
Overview
Mouse Eif4g2 encodes DAP-5 (also known as eIF4G2), a non-canonical translation initiation scaffold that supports cap-independent mRNA translation, particularly under stress conditions when cap-dependent translation is attenuated. DAP-5 interacts with components of the initiation machinery and contributes to IRES-mediated translation of select transcripts, shaping proteome output during apoptosis, ER stress, and nutrient deprivation. Through its role in translational control, DAP-5 influences cell survival decisions, cell-cycle progression, and differentiation programs. Dysregulation of EIF4G2/DAP-5–linked translational networks has been associated with contexts relevant to oncogenic transformation, neurobiology, and stress-adaptation phenotypes, making it a useful node for mechanistic studies.
DAP-5 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Eif4g2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Eif4g2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Eif4g2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DAP-5 protein expression.
This CRISPR knockout system enables efficient generation of Eif4g2-deficient cell models for investigation of DAP-5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Eif4g2 exon(s) critical for DAP-5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Eif4g2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DAP-5 CRISPR/Cas9 KO Plasmid (m) and DAP-5 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Eif4g2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DAP-5 HDR Plasmid (m) and DAP-5 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Eif4g2 homology arms to support homology-directed repair at defined Eif4g2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.