m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for Cytokeratin 15 Antibody (LHK15) for WB and IHC(P) applications. This reagent is now offered in a bundle with Cytokeratin 15 Antibody (LHK15) (see ordering information below).
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Cytokeratin 15 Antibody (LHK15) is a high quality monoclonal Cytokeratin 15 antibody (also designated KRT15 antibody, or Keratin 15 antibody) suitable for the detection of the Cytokeratin 15 protein of mouse, rat and human origin. Cytokeratin 15 Antibody (LHK15) is available as both the non-conjugated anti-Cytokeratin 15 antibody form, as well as multiple conjugated forms of anti-Cytokeratin 15 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cytokeratin 15 (CK15, K15, K1CO, keratin15, type I cytoskeletal 15) is an intermediate filament (IF) type I protein that is responsible for the mechanical integrity of epithelial cells. Keratin family members are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains, and are clustered in a region on chromosome 17q21.2. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceus. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Pricing up to buy this: 200ug/ml, but what volume?
Thank you for your question. This is provided as 200 ug in 1 ml volume.
回答者: Technical Support
Dear SAnta cruz,I wanted to try cytokeratin 15 antibody. But since its mouse antibody i am not sure if it will give me lot of background with my mouse skin sample.Is their any possibility to get free small aliquote for trail Please
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
回答者: SCBT Heidelberg Support
Can Cytokeratin 15 (LHK15): sc-47697 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?
Thank you for your question. Yes, Cytokeratin 15 (LHK15): sc-47697 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
works very well in histology alone or in coIFSerial sections from human scalp biopsies and mouse ears embedded in OCT or human scalp biopsies embedded in formalin‐fixed paraffin were processed for immunofluorescence as follow: Frozen sections were fixed with neutral buffered 4% paraformaldehyde (PFA) for 15 min at room temperature (RT) and washed three times with phosphate‐buffered saline (PBS). Paraffin sections were processed for their deparaffinization and antigen retrieval. Primary antibody specific for Keratin 15 at 1:400 dilution was incubated overnight (O/N) at 4°C. Sections were then washed three times with PBS and incubated with secondary antibody conjugated to the appropriate fluorophore(s) (for example, Alexa Fluor 647 dilution 1:200) for 1 h at RT. Sections were then washed three times with PBS, counterstained with DAPI, mounted with IF-grate antifade mounting medium and analyzed by fluorescence or confocal microscopy.
good antibodythe antibody was tested for flow cytometry in mammary cells. worked well.
Outstanding IHC stainingGreat product for IHC staining applications; also nice WB
Great IHC(P) dataThe Cytokeratin 15 Antibody (LHK15): sc-47697 works very well for IHC with formalin fixed, paraffin-embedded tissue sections. This antibody has been cited over 10 times for further verification.
Excellent images obtained in IF with humanExcellent images obtained in IF with human placenta -SCBT Publication Review
Works well in IHC with paraffinWorks well in IHC with paraffin-embedded human fetal corneal cells -SCBT Publication Review
Good single band with some backgroundGood single band with some background -SCBT QC