Cytokeratin 15抗体(LHK15) mouse, rat と human 由来のCytokeratin 15 WB, IP, IF と IHC(P)での検出にはお勧めします

Cytokeratin 15抗体(LHK15): sc-47697

Cytokeratin 15抗体(LHK15)は、 7件のレビューによって、 5つ星のうち 4.6と 評価されています。
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データシート

    • 反-Cytokeratin 15抗体 LHK15はマウスモノクローナルIgG2aCytokeratin 15 抗体 です。200 µg/mlで提供
    • raised against the last 17 amino acids of Cytokeratin 15 of human origin
    • 反-Cytokeratin 15抗体 (LHK15) mouse, rat と human 由来のCytokeratin 15 WB, IP, IF と IHC(P)での検出にはお勧めします
    • 反-Cytokeratin 15抗体 (LHK15) IP用のアガロース、に共役での利用可能です。WB、IHC(P)とELISA用のHRP、IF、IHC(P)とFCM用のphycoerythrin または FITC、に共役での利用可能です。
    • WB (RGB)、IF、IHC(P)、FCM、iBright™ FL1000、FluorChem™、Typhoon、Azureと他の同等システムでRGB蛍光イメージングシステム用のAlexa Fluor® 488Alexa Fluor® 546Alexa Fluor® 594 または Alexa Fluor® 647、に共役での利用可能です。
    • WB (NIR)、IF、FCMとLI-COR®/Odyssey®、iBright™ FL1000、FluorChem™、Typhoon、Azureと他の同等システムで近赤外(NIR)検出法用のAlexa Fluor® 680 または Alexa Fluor® 790、に共役での利用可能です。
    • Cytokeratin 15 (LHK15): sc-47697無料の10 µgサンプルを受け取るには、ご連絡ください。
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for Cytokeratin 15 Antibody (LHK15) for WB and IHC(P) applications. This reagent is now offered in a bundle with Cytokeratin 15 Antibody (LHK15) (see ordering information below).

Pricing up to buy this:  200ug/ml,  but what volume?

質問者: Shelly99
Thank you for your question. This is provided as 200 ug in 1 ml volume.
回答者: Technical Support
投稿日: 2020-03-25

Dear SAnta cruz,I wanted to try cytokeratin 15 antibody. But since its mouse antibody i am not sure if it will give me lot of background with my mouse skin sample.Is their any possibility to get free small aliquote for trail Please

質問者: zumer
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
回答者: SCBT Heidelberg Support
投稿日: 2018-12-14

Can Cytokeratin 15 (LHK15): sc-47697 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

質問者: jenni
Thank you for your question. Yes, Cytokeratin 15 (LHK15): sc-47697 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
回答者: Technical Support 8
投稿日: 2017-01-10
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によって、 5つ星のうち 5 と評価されています。 works very well in histology alone or in coIF Serial sections from human scalp biopsies and mouse ears embedded in OCT or human scalp biopsies embedded in formalin‐fixed paraffin were processed for immunofluorescence as follow: Frozen sections were fixed with neutral buffered 4% paraformaldehyde (PFA) for 15 min at room temperature (RT) and washed three times with phosphate‐buffered saline (PBS). Paraffin sections were processed for their deparaffinization and antigen retrieval. Primary antibody specific for Keratin 15 at 1:400 dilution was incubated overnight (O/N) at 4°C. Sections were then washed three times with PBS and incubated with secondary antibody conjugated to the appropriate fluorophore(s) (for example, Alexa Fluor 647 dilution 1:200) for 1 h at RT. Sections were then washed three times with PBS, counterstained with DAPI, mounted with IF-grate antifade mounting medium and analyzed by fluorescence or confocal microscopy.
投稿日: 2019-12-04
によって、 5つ星のうち 4 と評価されています。 good antibody the antibody was tested for flow cytometry in mammary cells. worked well.
投稿日: 2017-11-23
によって、 5つ星のうち 5 と評価されています。 Outstanding IHC staining Great product for IHC staining applications; also nice WB
投稿日: 2017-01-14
によって、 5つ星のうち 5 と評価されています。 Great IHC(P) data The Cytokeratin 15 Antibody (LHK15): sc-47697 works very well for IHC with formalin fixed, paraffin-embedded tissue sections. This antibody has been cited over 10 times for further verification.
投稿日: 2017-01-10
によって、 5つ星のうち 5 と評価されています。 Excellent images obtained in IF with human Excellent images obtained in IF with human placenta -SCBT Publication Review
投稿日: 2015-03-07
によって、 5つ星のうち 5 と評価されています。 Works well in IHC with paraffin Works well in IHC with paraffin-embedded human fetal corneal cells -SCBT Publication Review
投稿日: 2015-03-06
によって、 5つ星のうち 3 と評価されています。 Good single band with some background Good single band with some background -SCBT QC
投稿日: 2015-02-15
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