COX4 Antibody (4D11-B3-E8): sc-517553

COX4 Antibody (4D11-B3-E8) is a mouse monoclonal IgG antibody that detects COX4 in mouse, rat, and human samples through applications such as western blotting (WB), immunofluorescence (IF), and immunohistochemistry. Anti-COX4 antibody (4D11-B3-E8) is available in a non-conjugated form, providing flexibility for various experimental needs. Cytochrome c oxidase (COX) serves as the terminal oxidase in the mitochondrial respiratory chain, utilizing cytochrome c as an electron donor to establish a proton gradient across the inner mitochondrial membrane, which is crucial for ATP production. The mammalian COX enzyme is a heteromer composed of three catalytic subunits encoded by mitochondrial DNA and several structural subunits encoded by nuclear genes, highlighting the intricate interplay between mitochondrial and nuclear genomes in cellular respiration. COX4, a nuclear-encoded subunit of COX, is essential for the regulation of COX activity and is expressed widely across adult human tissues, with the highest expression levels found in the pancreas and moderate levels in the heart, skeletal muscle, and placenta. Understanding COX4′s role in cellular respiration and expression patterns can provide insights into metabolic disorders and mitochondrial dysfunction, making COX4 (4D11-B3-E8) monoclonal antibody a valuable tool for researchers studying these critical biological processes.

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COX4 Antibody (4D11-B3-E8) References:

1. The 5' region of the COX4 gene contains a novel overlapping gene, NOC4.  |  Bachman, NJ., et al. 1999. Mamm Genome. 10: 506-12. PMID: 10337626
2. Cloning of COX4 cDNA from the NZ white rabbit and expression of this gene in bladder smooth muscle following partial outlet obstruction.  |  Wu, X., et al. 2002. World J Urol. 20: 255-9. PMID: 12215857
3. The characterization and role of zinc binding in yeast Cox4.  |  Coyne, HJ., et al. 2007. J Biol Chem. 282: 8926-34. PMID: 17215247
4. Polyamine modulon in yeast-Stimulation of COX4 synthesis by spermidine at the level of translation.  |  Uemura, T., et al. 2009. Int J Biochem Cell Biol. 41: 2538-45. PMID: 19695341
5. The cardiac copper chaperone proteins Sco1 and CCS are up-regulated, but Cox 1 and Cox4 are down-regulated, by copper deficiency.  |  Getz, J., et al. 2011. Biol Trace Elem Res. 143: 368-77. PMID: 20878365
6. Evaluating the role of NRF-1 in the regulation of the goldfish COX4-1 gene in response to temperature.  |  Gao, G. and Moyes, CD. 2016. J Exp Biol. 219: 3019-3027. PMID: 27471277
7. Repositioning chlorpromazine for treating chemoresistant glioma through the inhibition of cytochrome c oxidase bearing the COX4-1 regulatory subunit.  |  Oliva, CR., et al. 2017. Oncotarget. 8: 37568-37583. PMID: 28455961
8. Cytochrome C Oxidase Subunit 4 (COX4): A Potential Therapeutic Target for the Treatment of Medullary Thyroid Cancer.  |  Bikas, A., et al. 2020. Cancers (Basel). 12: PMID: 32911610
9. Upregulation of COX4-2 via HIF-1α in Mitochondrial COX4-1 Deficiency.  |  Douiev, L., et al. 2021. Cells. 10: PMID: 33672589
10. Replicative Stress Coincides with Impaired Nuclear DNA Damage Response in COX4-1 Deficiency.  |  Douiev, L., et al. 2022. Int J Mol Sci. 23: PMID: 35456968

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-COX4 Antibody (4D11-B3-E8) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-COX4 Antibody (4D11-B3-E8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

COX4 Antibody (4D11-B3-E8) Specifications

Alternate Product Name: COX4

Product Clone Name: 4D11-B3-E8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG

Amino Acids:

Epitope Target: COX4

Epitope Species: human

Product Reactivity/Detection: COX4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: hamster, goat and monkey

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF, IHC(P)

Vialing Information: Each vial contains 100 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, 1% glycerol and < 0.1% stabilizer protein

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for COX4 Antibody (4D11-B3-E8): N/A

The preferred COX4 antibody is COX4 Antibody (F-8): sc-376731.