CKIP-1 Antibody (A-3): sc-376060

CKIP-1 Antibody (A-3) is a mouse monoclonal IgG1 antibody that detects Casein Kinase II-interacting protein 1 (CKIP-1) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CKIP-1, also known as pleckstrin homology domain containing family O member 1 (PLEKHO1), is a 409-amino acid protein characterized by an N-terminal pleckstrin homology domain and a putative C-terminal JUN leucine zipper interactive domain. CKIP-1 plays a crucial role in cellular signaling pathways, particularly in the regulation of protein kinase CK2, which is involved in various cellular processes such as cell growth, differentiation, and apoptosis. CKIP-1 is predominantly expressed in skeletal muscle and heart tissues, with intermediate levels found in the placenta, lung, and brain, while expression is minimal in the pancreas, liver, and kidney. CKIP-1 localizes to the plasma membrane and nucleus in human osteosarcoma cells, suggesting a role in both membrane-associated signaling and nuclear functions, potentially influencing gene expression. CKIP-1 interacts specifically with the N terminus of CSNK2A1 and the full-length CSNK2A1, but does not interact with CSNK2A2 or CSNK2B, highlighting specificity in protein-protein interactions vital for cellular signaling.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CKIP-1 Antibody (A-3) References:

1. Physiological functions of CKIP-1: From molecular mechanisms to therapy implications.  |  Fu, L. and Zhang, L. 2019. Ageing Res Rev. 53: 100908. PMID: 31082489
2. CKIP-1 augments autophagy in steatotic hepatocytes by inhibiting Akt/mTOR signal pathway.  |  Li, L., et al. 2020. Exp Cell Res. 397: 112341. PMID: 33191205
3. Ckip-1 regulates C3H10T1/2 mesenchymal cell proliferation and osteogenic differentiation via Lrp5.  |  Huang, X., et al. 2021. Exp Ther Med. 21: 342. PMID: 33732315
4. CKIP-1 contributes to osteogenic differentiation of mouse bone marrow mesenchymal stem cells.  |  Niu, Q., et al. 2021. Regen Med. 16: 847-859. PMID: 34498492
5. Ckip-1 Mediates P. gingivalis-Suppressed Cementoblast Mineralization.  |  Huang, X., et al. 2022. J Dent Res. 101: 599-608. PMID: 34875910
6. CKIP-1 Promotes P. gingivalis-Induced Inflammation of Periodontal Soft Tissues by Inhibiting Autophagy.  |  Xiao, J., et al. 2023. Inflammation. 46: 1997-2010. PMID: 37351817
7. Ckip-1 3'UTR alleviates prolonged sleep deprivation induced cardiac dysfunction by activating CaMKK2/AMPK/cTNI pathway.  |  Dong, B., et al. 2024. Mol Biomed. 5: 23. PMID: 38871861
8. CKIP-1-Loaded Cartilage-Affinitive Nanoliposomes Reverse Osteoarthritis by Restoring Chondrocyte Homeostasis.  |  Cao, F., et al. 2024. ACS Biomater Sci Eng. 10: 4437-4451. PMID: 38885017
9. CKIP-1 silencing suppresses OSCC via mitochondrial homeostasis-associated TFAM/cGAS-STING signalling axis.  |  Xie, JR., et al. 2024. J Cell Mol Med. 28: e70006. PMID: 39169452
10. The Discovery of a Specific CKIP-1 Ligand for the Potential Treatment of Disuse Osteoporosis.  |  Wei, Y., et al. 2024. Int J Mol Sci. 25: PMID: 39201556

The preferred CKIP-1 antibody is CKIP-1 Antibody (A-12): sc-376355.