CHST1 Antibody (E-3): sc-271599
- CHST1 Antibody (E-3) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 305-411 mapping at the C-terminus of CHST1 of human origin
- recommended for detection of CHST1 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CHST1 Antibody (E-3) for WB applications. These reagents are now offered in bundles with CHST1 Antibody (E-3) (see ordering information below).
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CHST1 Antibody (E-3) is a mouse monoclonal IgG2a antibody that detects CHST1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CHST1, also known as KSGal6ST or KSST, is a keratan sulfate sulfotransferase that plays a crucial role in the sulfation of keratan in the cornea, which is vital for maintaining corneal transparency. CHST1 is predominantly expressed in the brain and skeletal muscle, where CHST1 localizes to the trans-Golgi network, facilitating the transfer of sulfate groups from 3′-phosphoadenosine 5′-phosphosulfate to specific galactose residues on keratan sulfate chains. Proper functioning of CHST1 is essential not only for corneal health but also for the generation of L-selectin ligands, which are important for leukocyte trafficking. Mutations in the CHST1 gene have been implicated in macular corneal dystrophy, highlighting the significance of CHST1 in both normal physiology and disease states. anti-CHST1 antibody (E-3) is an invaluable tool for researchers studying the role of sulfotransferases in various biological processes.
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CHST1 Antibody (E-3) References:
- CHST1 and CHST2 sulfotransferases expressed by human vascular endothelial cells: cDNA cloning, expression, and chromosomal localization. | Li, X. and Tedder, TF. 1999. Genomics. 55: 345-7. PMID: 10049591
- CHST1 and CHST2 sulfotransferase expression by vascular endothelial cells regulates shear-resistant leukocyte rolling via L-selectin. | Li, X., et al. 2001. J Leukoc Biol. 69: 565-74. PMID: 11310842
- Catalog of 77 single-nucleotide polymorphisms (SNPs) in the carbohydrate sulfotransferase 1 (CHST1) and carbohydrate sulfotransferase 3 (CHST3) genes. | Iida, A., et al. 2002. J Hum Genet. 47: 14-9. PMID: 11829137
- Characterization of expression of glycan ligands for Siglec-F in normal mouse lungs. | Guo, JP., et al. 2011. Am J Respir Cell Mol Biol. 44: 238-43. PMID: 20395633
- Airway glycomic and allergic inflammatory consequences resulting from keratan sulfate galactose 6-O-sulfotransferase (CHST1) deficiency. | Kumagai, T., et al. 2018. Glycobiology. 28: 406-417. PMID: 29659839
- Probing the binding specificities of human Siglecs by cell-based glycan arrays. | Büll, C., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 33893239
- Exploring the sulfate patterns of chondroitin sulfate/dermatan sulfate and keratan sulfate in human pancreatic cancer. | Ren, Q., et al. 2021. J Pharm Biomed Anal. 205: 114339. PMID: 34464868
- Genome-wide DNA methylation profiling in anorexia nervosa discordant identical twins. | Iranzo-Tatay, C., et al. 2022. Transl Psychiatry. 12: 15. PMID: 35013117
- ACAN, MDFI, and CHST1 as Candidate Genes in Gastric Cancer: A Comprehensive Insilco Analysis. | Vafaeie, F., et al. 2022. Asian Pac J Cancer Prev. 23: 683-694. PMID: 35225482
- Exploiting Substrate Specificities of 6-O-Sulfotransferases to Enzymatically Synthesize Keratan Sulfate Oligosaccharides. | Wu, Y., et al. 2023. JACS Au. 3: 3155-3164. PMID: 38034954
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CHST1 Antibody (E-3) | sc-271599 | 200 µg/ml | $322.00 | |||
CHST1 Antibody (E-3): m-IgG Fc BP-HRP Bundle | sc-537667 | 200 µg Ab; 10 µg BP | $361.00 | |||
CHST1 Antibody (E-3): m-IgGκ BP-HRP Bundle | sc-535006 | 200 µg Ab; 40 µg BP | $361.00 |