Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Chk2 CRISPR/Cas9 KO Plasmid (m): sc-424521
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Chk2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Chk2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Chk2 Antibody (A-11): sc-17747
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Chk2 CRISPR/Cas9 KO Plasmid (m) | sc-424521 | 20 µg | $397.00 |
Overview
Chek2 encodes the checkpoint kinase Chk2, a serine/threonine kinase activated downstream of ATM in response to DNA double-strand breaks. Once phosphorylated, Chk2 propagates DNA damage signaling by modulating effectors such as p53 and CDC25 phosphatases, thereby influencing cell-cycle checkpoint control, DNA repair pathway choice, senescence, and apoptosis. In mouse systems, Chek2 activity is commonly interrogated in studies of genome stability, replication stress, and oxidative damage responses. Dysregulation of CHEK2/Chk2 signaling is broadly relevant to malignancy-associated DNA damage response defects and can alter sensitivity to genotoxic stress in experimental models.
Chk2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Chek2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Chek2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Chek2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Chk2 protein expression.
This CRISPR knockout system enables efficient generation of Chek2-deficient cell models for investigation of Chk2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Chek2 exon(s) critical for Chk2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Chek2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Chk2 CRISPR/Cas9 KO Plasmid (m) and Chk2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Chek2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Chk2 HDR Plasmid (m) and Chk2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Chek2 homology arms to support homology-directed repair at defined Chek2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.