Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Centriolin CRISPR/Cas9 KO Plasmid (h): sc-404195
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Centriolin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Centriolin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Centriolin Antibody (C-9): sc-365521
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Centriolin CRISPR/Cas9 KO Plasmid (h) | sc-404195 | 20 µg | $397.00 |
Overview
CNTRL encodes centriolin, a centrosome- and midbody-associated scaffold protein required for centrosome maturation, mitotic progression, and completion of cytokinesis. Centriolin supports recruitment and organization of protein complexes at the mother centriole and midbody, coordinating microtubule-based structures that ensure proper spindle function and abscission. Disruption of CNTRL can perturb centrosome homeostasis, lead to cytokinesis failure, and promote chromosomal instability, processes relevant to studying aneuploidy and proliferative stress in human cell models. CNTRL is therefore a useful node for investigating centrosome regulation, cell-cycle checkpoints, and division-plane control.
Centriolin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CNTRL gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CNTRL together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CNTRL open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Centriolin protein expression.
This CRISPR knockout system enables efficient generation of CNTRL-deficient cell models for investigation of Centriolin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CNTRL exon(s) critical for Centriolin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CNTRL genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Centriolin CRISPR/Cas9 KO Plasmid (h) and Centriolin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CNTRL locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Centriolin HDR Plasmid (h) and Centriolin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CNTRL homology arms to support homology-directed repair at defined CNTRL target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.