m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for CDKAL1 Antibody (E-9) for WB applications. This reagent is now offered in a bundle with CDKAL1 Antibody (E-9) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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CDKAL1 Antibody (E-9) is a high quality monoclonal CDKAL1 antibody (also designated CDKAL1 antibody) suitable for the detection of the CDKAL1 protein of mouse, rat and human origin. CDKAL1 Antibody (E-9) is available as both the non-conjugated anti-CDKAL1 antibody form, as well as multiple conjugated forms of anti-CDKAL1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cell cycle progression is controlled, in part, by a family of cyclin dependent kinases (Cdks) that work to phosphorylate key substrates involved in each phase of cell cycle progression. Cdks are the catalytic subunits of serine/threonine protein kinases, a large family of proteins that act as regulators of the eukaryotic cell cycle. CDKAL1 (Cdk5 regulatory subunit associated protein 1-like 1) is a 579 amino acid single-pass membrane protein that contains one TRAM domain and is similar to Cdk5 regulatory subunit associated proteins (CDK5RAPs). Expressed in pancreas, brain and skeletal muscle, CDKAL1 uses iron as a cofactor and is involved in glucose-stimulated insulin secretion. Defects in the gene encoding CDKAL1 impair insulin secretion and are associated with the development of type 2 diabetes. Multiple isoforms of CDKAL1 exist due to alternative splicing events.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Do you have a recombinant Cdkal1 protein to use as a standard in ELISA when using the E9 Cdkal 1 primary antibody?
Thank you for your question. No, we do not have a recombinant CDKAL1 protein that is commercially available.
回答者: Technical Support
I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with CDKAL1 (E-9): sc-393447 monoclonal antibody?
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGλ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here:
Very good Western blot data of CDKAL1 expressionVery good Western blot data of CDKAL1 expression in Hep G2, U-2 OS and Jurkat whole cell lysates and human skeletal muscle and human pancreas tissue extracts. -SCBT QC