Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD88 CRISPR/Cas9 KO Plasmid (m): sc-419395
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD88 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD88 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD88 Antibody (R63): sc-53797
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD88 CRISPR/Cas9 KO Plasmid (m) | sc-419395 | 20 µg | $397.00 |
Overview
C5ar1 encodes CD88, the canonical G protein–coupled receptor for the complement anaphylatoxin C5a, and is highly expressed on myeloid lineage cells including neutrophils, monocytes, and macrophages. Upon ligand engagement, CD88 activates Gi-dependent signaling that drives chemotaxis, calcium mobilization, MAPK and PI3K pathways, oxidative burst, and cytokine production, integrating complement cues with innate immune activation. This receptor participates in inflammatory amplification and leukocyte recruitment, intersecting with Fc receptor signaling and pattern-recognition pathways to shape tissue injury and repair responses. Dysregulated C5a–CD88 signaling has been implicated in models of sepsis, autoimmune inflammation, ischemia-reperfusion injury, and neuroinflammation, making C5ar1 a useful node for mechanistic studies in immunopathology.
CD88 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the C5ar1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the C5ar1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the C5ar1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD88 protein expression.
This CRISPR knockout system enables efficient generation of C5ar1-deficient cell models for investigation of CD88 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting C5ar1 exon(s) critical for CD88 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple C5ar1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD88 CRISPR/Cas9 KO Plasmid (m) and CD88 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the C5ar1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD88 HDR Plasmid (m) and CD88 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by C5ar1 homology arms to support homology-directed repair at defined C5ar1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.