CD3-ζ Antibody (D-4): sc-374281
- CD3-ζ Antibody (D-4) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-163 representing full length CD3-ζ of human origin
- Anti-CD3-zeta Antibody (D-4) is recommended for detection of CD3-ζ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-CD3-zeta Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD3-ζ Antibody (D-4) for WB and IHC(P) applications. These reagents are now offered in bundles with CD3-ζ Antibody (D-4) (see ordering information below).
CD3-ζ Antibody (D-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD3-ζ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CD3-ζ (D-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD3-ζ protein plays a crucial role in T cell activation, as CD3-ζ is part of the T cell receptor (TCR) complex that transmits signals upon recognition of foreign antigens. This signaling is essential for the immune response, as CD3-ζ leads to the activation of T cells, which are vital for adaptive immunity. The CD3 complex, which includes CD3-ζ, consists of five invariant polypeptide chains that form three distinct dimers, facilitating the transmission of activation signals. The ζ chain contains three immunoreceptor tyrosine-based activation motifs (ITAMs), which are critical for recruiting protein kinases like ZAP-70 and Syk, thereby amplifying the signaling cascade that results in T cell activation. The structural integrity and phosphorylation state of these motifs are vital for effective immune responses, making CD3-ζ a key player in immunological signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD3-ζ Antibody (D-4) | sc-374281 | 200 µg/ml | $322.00 | |||
CD3-ζ Antibody (D-4): m-IgG Fc BP-HRP Bundle | sc-529709 | 200 µg Ab; 10 µg BP | $361.00 | |||
CD3-ζ Antibody (D-4): m-IgGκ BP-HRP Bundle | sc-522685 | 200 µg Ab, 40 µg BP | $361.00 | |||
CD3-ζ Antibody (D-4) AC | sc-374281 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CD3-ζ Antibody (D-4) HRP | sc-374281 HRP | 200 µg/ml | $322.00 | |||
CD3-ζ Antibody (D-4) FITC | sc-374281 FITC | 200 µg/ml | $336.00 | |||
CD3-ζ Antibody (D-4) PE | sc-374281 PE | 200 µg/ml | $349.00 | |||
CD3-ζ Antibody (D-4) Alexa Fluor® 488 | sc-374281 AF488 | 200 µg/ml | $364.00 | |||
CD3-ζ Antibody (D-4) Alexa Fluor® 546 | sc-374281 AF546 | 200 µg/ml | $364.00 | |||
CD3-ζ Antibody (D-4) Alexa Fluor® 594 | sc-374281 AF594 | 200 µg/ml | $364.00 | |||
CD3-ζ Antibody (D-4) Alexa Fluor® 647 | sc-374281 AF647 | 200 µg/ml | $364.00 | |||
CD3-ζ Antibody (D-4) Alexa Fluor® 680 | sc-374281 AF680 | 200 µg/ml | $364.00 | |||
CD3-ζ Antibody (D-4) Alexa Fluor® 790 | sc-374281 AF790 | 200 µg/ml | $364.00 |