Calpain 1 Antibody (A-5): sc-390677
- Calpain 1 Antibody (A-5) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 682-709 of Calpain 1 catalytic subunit of human origin
- recommended for detection of Calpain 1 catalytic subunit of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- See Calpain 1 (D-11): sc-271313 for Calpain 1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Calpain 1 Antibody (A-5) for WB applications. These reagents are now offered in bundles with Calpain 1 Antibody (A-5) (see ordering information below).
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Calpain 1 Antibody (A-5) is a mouse monoclonal IgG1 antibody that detects Calpain 1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Calpain 1, also known as µ-calpain, is an intracellular calcium-dependent protease that plays a crucial role in various cellular processes by cleaving cytoskeletal and submembranous proteins. This protein is essential for mediating calcium-dependent events such as cell fusion, mitosis, and meiosis, which are vital for normal cellular function and development. The structure of Calpain 1 is characterized by a heterodimeric composition, consisting of a small regulatory subunit and a large catalytic subunit, which allows for precise regulation of proteolytic activity. Regulation of Calpain 1 is further influenced by calpastatin, a specific inhibitor that modulates activity and membrane binding, highlighting the importance of post-translational modifications in controlling function. Notably, Calpain 1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain, suggesting involvement in neuroinflammatory processes. Additionally, Calpain 1 is critical in spermatogenesis and the acrosome reaction, underscoring significance in reproductive biology. With versatile applications and essential biological roles, anti-Calpain 1 antibody (A-5) serves as an invaluable tool for researchers studying cellular dynamics and signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Calpain 1 Antibody (A-5) References:
- Calpain-1 contributes to IgE-mediated mast cell activation. | Wu, Z., et al. 2014. J Immunol. 192: 5130-9. PMID: 24760147
- Role of calpain-1 in the early phase of experimental ALS. | Stifanese, R., et al. 2014. Arch Biochem Biophys. 562: 1-8. PMID: 25151305
- Physiological Roles of Calpain 1 Associated to Multiprotein NMDA Receptor Complex. | Averna, M., et al. 2015. PLoS One. 10: e0139750. PMID: 26431040
- Clinical correlation of calpain-1 and glypican-3 expression with gallbladder carcinoma. | Luo, W., et al. 2016. Oncol Lett. 11: 1345-1352. PMID: 26893741
- Calpain-1 regulates platelet function in a humanized mouse model of sickle cell disease. | Nwankwo, JO., et al. 2017. Thromb Res. 160: 58-65. PMID: 29101791
- Inhibition of calpain-1 stabilizes TCF11/Nrf1 but does not affect its activation in response to proteasome inhibition. | Nowak, K., et al. 2018. Biosci Rep. 38: PMID: 30177525
- Calpain 1 in bronchoalveolar lavage fluid is associated with poor prognosis in lepidic predominant pulmonary adenocarcinoma. | Ruppert, AM., et al. 2019. Bull Cancer. 106: 179-188. PMID: 30683309
- Synthesis of α-Ketoamide-Based Stereoselective Calpain-1 Inhibitors as Neuroprotective Agents. | Jastaniah, A., et al. 2020. ChemMedChem. 15: 2280-2285. PMID: 32840034
- Tetrandrine alleviates podocyte injury via calcium-dependent calpain-1 signaling blockade. | Ding, Y., et al. 2021. BMC Complement Med Ther. 21: 296. PMID: 34906112
- The Correlation of Serum Calpain 1 Activity and Concentrations of Interleukin 33 in COVID-19 Acute Respiratory Distress Syndrome. | Loinjak, D., et al. 2023. Biomedicines. 11: PMID: 37509486
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Calpain 1 Antibody (A-5) | sc-390677 | 200 µg/ml | $322.00 | |||
Calpain 1 Antibody (A-5): m-IgG Fc BP-HRP Bundle | sc-540832 | 200 µg Ab; 10 µg BP | $361.00 | |||
Calpain 1 Antibody (A-5): m-IgG1 BP-HRP Bundle | sc-542401 | 200 µg Ab; 20 µg BP | $361.00 | |||
Calpain 1 (A-5) Neutralizing Peptide | sc-390677 P | 100 µg/0.5 ml | $69.00 |