C6 Antibody (D-8): sc-390735
- C6 Antibody (D-8) is a mouse monoclonal IgG2b κ C6 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 118-360 mapping near the N-terminus of C6 of human origin
- C6 Antibody (D-8) is recommended for detection of C6 of human origin by WB, IP, IF and ELISA
- Anti-C6 Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for C6 Antibody (D-8) for WB applications. This reagent is now offered in a bundle with C6 Antibody (D-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
C6 Antibody (D-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects C6 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-C6 antibody (D-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. C6 protein, consisting of 934 amino acids, is a crucial component of the complement system, which is integral to the innate immune response. Specifically, C6 is involved in the formation of the membrane attack complex, a structure that can insert into the membranes of pathogens, leading to cell lysis and elimination of infections. C6 is expressed as two transcript variants and features several important structural domains, including one EGF-like domain, one LDL-receptor class A domain, one MACPF domain, two Sushi domains, and three TSP type-1 domains. Notably, deficiencies in C6 are associated with an increased susceptibility to bacterial infections, underscoring its vital role in immune defense. Using C6 (D-8) monoclonal antibody in research can provide insights into the mechanisms of the complement cascade and its implications in various immunological conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
C6 Antibody (D-8) References:
- Complement membrane attack is required for endplate damage and clinical disease in passive experimental myasthenia gravis in Lewis rats. | Chamberlain-Banoub, J., et al. 2006. Clin Exp Immunol. 146: 278-86. PMID: 17034580
- Antibody and complement mediated injury in transplants following sensitization by allogeneic blood transfusion. | Qian, Z., et al. 2006. Transplantation. 82: 857-64. PMID: 17038898
- Prevalence of mutations leading to complete C6 deficiency (C6Q0) in the Western Cape, South Africa and detection of novel mutations leading to C6Q0 in an Irish family. | Parham, KL., et al. 2007. Mol Immunol. 44: 2756-60. PMID: 17257682
- Selective therapeutic control of C5a and the terminal complement complex by anti-C5 single-chain Fv in an experimental model of antigen-induced arthritis in rats. | Fischetti, F., et al. 2007. Arthritis Rheum. 56: 1187-97. PMID: 17393439
- Re-establishment of complement C6-deficient rabbit colony by cryopreserved sperm transported from abroad. | Liu, E., et al. 2007. Exp Anim. 56: 167-71. PMID: 17460363
- The molecular architecture of human complement component C6. | DiScipio, RG. and Hugli, TE. 1989. J Biol Chem. 264: 16197-206. PMID: 2789218
- Complete primary structure and functional characterization of the sixth component of the human complement system. Identification of the C5b-binding domain in complement C6. | Haefliger, JA., et al. 1989. J Biol Chem. 264: 18041-51. PMID: 2808363
- Structure of the human C6 gene. | Hobart, MJ., et al. 1993. Biochemistry. 32: 6198-205. PMID: 8512929
- Characterization of the human C6 promoter: requirement of the CCAAT enhancer binding protein binding site for C6 gene promoter activity. | González, S. and López-Larrea, C. 1996. J Immunol. 157: 2282-90. PMID: 8805625
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C6 Antibody (D-8) | sc-390735 | 200 µg/ml | $322.00 | |||
C6 Antibody (D-8): m-IgGκ BP-HRP Bundle | sc-523657 | 200 µg Ab, 40 µg BP | $361.00 | |||
C6 Antibody (D-8) AC | sc-390735 AC | 500 µg/ml, 25% agarose | $424.00 | |||
C6 Antibody (D-8) HRP | sc-390735 HRP | 200 µg/ml | $322.00 | |||
C6 Antibody (D-8) FITC | sc-390735 FITC | 200 µg/ml | $336.00 | |||
C6 Antibody (D-8) PE | sc-390735 PE | 200 µg/ml | $349.00 | |||
C6 Antibody (D-8) Alexa Fluor® 488 | sc-390735 AF488 | 200 µg/ml | $364.00 | |||
C6 Antibody (D-8) Alexa Fluor® 546 | sc-390735 AF546 | 200 µg/ml | $364.00 | |||
C6 Antibody (D-8) Alexa Fluor® 594 | sc-390735 AF594 | 200 µg/ml | $364.00 | |||
C6 Antibody (D-8) Alexa Fluor® 647 | sc-390735 AF647 | 200 µg/ml | $364.00 | |||
C6 Antibody (D-8) Alexa Fluor® 680 | sc-390735 AF680 | 200 µg/ml | $364.00 | |||
C6 Antibody (D-8) Alexa Fluor® 790 | sc-390735 AF790 | 200 µg/ml | $364.00 |