Date published: 2025-9-6

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C3抗体(B-9): sc-28294

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データシート
  • C3抗体 B-9はマウスモノクローナルIgG1C3 抗体 です。200 µg/mlで提供
  • human由来のC3 precursorのアミノ酸541-840に対応します
  • C3抗体 (B-9) mouse, rat と human 由来のC3 precursor, C3a anaphylatoxin, C3 α chain, C3 β chain and C3b α′ chain WB, IP, IF, IHC(P) と ELISAでの検出にはお勧めします
  • 抗 C3 抗体 (B-9) は、IP 用には アガロース、WB、IHC(P)、ELISA 用には HRP、IF、IHC(P)、FCM 用には フィコエリスリン または FITC にそれぞれ結合したものが利用可能
  • WB (RGB)、IF、IHC(P)、FCM、iBright™ FL1000、FluorChem™、Typhoon、Azureと他の同等システムでRGB蛍光イメージングシステム用のAlexa Fluor® 488Alexa Fluor® 546Alexa Fluor® 594 または Alexa Fluor® 647、に共役での利用可能です。
  • WB (NIR)、IF、FCMとLI-COR®/Odyssey®、iBright™ FL1000、FluorChem™、Typhoon、Azureと他の同等システムで近赤外(NIR)検出法用のAlexa Fluor® 680 または Alexa Fluor® 790、に共役での利用可能です。
  • C3 (B-9): sc-28294無料の10 µgサンプルを受け取るには、ご連絡ください。
  • m-IgG Fc BP-HRP and m-IgGκ BP-HRP は C3 抗体 (B-9) に推奨される二次検出試薬であり、 WB and IHC(P) アプリケーションに用いられる。この試薬は只今、 C3 抗体 (B-9) とセットとしても提供されます。 (下記の注文情報をご覧ください)。

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関連項目

C3 Antibody (B-9) は、ヒト補体成分3(C3)タンパクのアミノ酸541-840に対して作られたマウスモノクローナルIgG1κ軽鎖抗体です。C3は、CPAMD1またはアシル化刺激タンパク(ASP)としても知られ、補体系の中心的な構成成分であり、先天性免疫において重要な役割を果たしています。主に肝臓で合成されたC3は血流に分泌され、細胞外空間に存在し、不活性前駆体として循環します。活性化されると、C3はC3aとC3bの断片に切断されます。C3aは炎症反応を媒介するアナフィラトキシンとして機能し、C3bは病原体の表面に結合し、オプソニン化と免疫細胞による貪食を促進します。C3は細胞外に存在することが不可欠であり、侵入してきた病原体と即座に相互作用し、細胞表面で補体カスケードを活性化することができます。C3の活性化の制御が失われると、自己免疫疾患や感染症にかかりやすくなる可能性があります。C3 (B-9) 抗体は、マウス、ラット、ヒト由来のC3前駆体、C3a アナフィラトキシン、C3 α 鎖、C3 β 鎖、C3b α′ 鎖を認識し、ウェスタンブロッティング (WB)、免疫沈降 (IP)、免疫蛍光 (IF)、パラフィン包埋切片を用いた免疫組織化学 (IHCP)、酵素免疫測定法 (ELISA) での使用が推奨されます。C3 (B-9) モノクローナル抗体は、補体活性化経路と、複数の種にわたる免疫反応におけるその関連性を研究するための貴重なツールです。

試験・研究用以外には使用しないでください。 臨床及び体外診断には使用できません。

Alexa Fluor® はMolecular Probes Inc., OR., USAの商標です。

LI-COR® and Odyssey® はLI-COR Biosciencesの登録商標です。

C3抗体(B-9) 参考文献:

  1. C3a活性の調節:ヒトC3aレセプターの内在化とC5aによる阻害。  |  Settmacher, B., et al. 1999. J Immunol. 162: 7409-16. PMID: 10358194
  2. アナフィラトキシン受容体C3aRとC5aRのリガンド誘導性リン酸化は, 「Gタンパク質共役型受容体キナーゼ」によって媒介される。  |  Langkabel, P., et al. 1999. Eur J Immunol. 29: 3035-46. PMID: 10508278
  3. 喘息のエフェクター期におけるC3aアナフィラトキシン受容体の役割。  |  Humbles, AA., et al. 2000. Nature. 406: 998-1001. PMID: 10984054
  4. ヒト補体成分C3: cDNAコード配列と一次構造.  |  de Bruijn, MH. and Fey, GH. 1985. Proc Natl Acad Sci U S A. 82: 708-12. PMID: 2579379
  5. Mitogen-activated protein kinaseの活性化には, ヒトアナフィラトキシンC5a受容体からの2つのシグナル入力が必要である。  |  Buhl, AM., et al. 1995. J Biol Chem. 270: 19828-32. PMID: 7649993
  6. 実験的リステリア髄膜脳炎におけるTNFαを介したアナフィラトキシンC5aの神経細胞上での受容体発現。  |  Stahel, PF., et al. 1997. J Immunol. 159: 861-9. PMID: 9218605

注文情報

製品名カタログ #単位価格数量お気に入り

C3 抗体 (B-9)

sc-28294
200 µg/ml
$316.00

C3 (B-9): m-IgG Fc BP-HRP Bundle

sc-528410
200 µg Ab; 10 µg BP
$354.00

C3 (B-9): m-IgGκ BP-HRP Bundle

sc-520784
200 µg Ab, 40 µg BP
$354.00

C3 抗体 (B-9) AC

sc-28294 AC
500 µg/ml, 25% agarose
$416.00

C3 抗体 (B-9) HRP

sc-28294 HRP
200 µg/ml
$316.00

C3 抗体 (B-9) FITC

sc-28294 FITC
200 µg/ml
$330.00

C3 抗体 (B-9) PE

sc-28294 PE
200 µg/ml
$343.00

C3 抗体 (B-9) Alexa Fluor® 488

sc-28294 AF488
200 µg/ml
$357.00

C3 抗体 (B-9) Alexa Fluor® 546

sc-28294 AF546
200 µg/ml
$357.00

C3 抗体 (B-9) Alexa Fluor® 594

sc-28294 AF594
200 µg/ml
$357.00

C3 抗体 (B-9) Alexa Fluor® 647

sc-28294 AF647
200 µg/ml
$357.00

C3 抗体 (B-9) Alexa Fluor® 680

sc-28294 AF680
200 µg/ml
$357.00

C3 抗体 (B-9) Alexa Fluor® 790

sc-28294 AF790
200 µg/ml
$357.00

Hello-Which antibody pairs would you recommend to develop a C3 sandwich ELISA

質問者: Aaron Stoker
Thank you for your question. We have not validated our antibodies for sandwich ELISA.
回答者: BlakeJ
投稿日: 2024-09-20

hello,i see C3 antibody (B-9): sc-28294 is a mouse monoclonal antibody,how can it detect c3a anaphylatoxin and C3b α′ chain at the same time,if i want to use it in IHC,how can i distinguish it is C3b,C3a or c3?

質問者: sophia
Thank you for your question. C3 Antibody (B-9) is recommended for detection of C3 precursor, C3a anaphylatoxin, C3 α chain, C3 β chain and C3b α′ chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. If you would like to detect the specific protein, please select the other antibody. Please contact our Technical Service Department if you have any other questions. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
回答者: Sen Li
投稿日: 2023-12-25

hello,can C3 Antibody (B-9): sc-28294 be used to dectect C3a anaphylatoxin in mouse.If so,did you have any such successful examples (western images) to shown to me ?

質問者: sophia0314
Thank you for your question. Yes, C3 Antibody (B-9): sc-28294 is recommended for detection of C3a anaphylatoxin in mouse sample by WB, IP, IF, IHC(P) and ELISA. We don't have an in-house data for detection of C3a anaphylatoxin in mouse sample by WB at this moment, but please refer to the citations for this product.
回答者: Technical Service SH
投稿日: 2023-03-16

Can I use non-transfected HEK-293T as a negative control? In your legend of Near-infrared western blot analysis of C3 expression said 293T has C3 band. But same time in your "sc-28294 HRP. Direct western blot analysis" legend said 293T has no band.

質問者: Woosung
Thank you for your question. Our Control 293T Lysate: sc-117752 is derived from non-transfected 293T cells and suitable for use as a negative control with our 293T Transfected Lysates. Our Control 293T Lysate goes through the same transfection process as our gene-specific transfected lysates, except without transfecting any cDNA into the cells. The Control 293T Lysate is also used at a lower concentration than regular cell lines for Western blotting. That said, it does appear that HEK293 does not have significant RNA expression of C3 per the Human Protein Atlas. We will review of the Near-infrared Western blot data showing a signal in HEK293T cell lysate, and if it can not be supported we will remove it.
回答者: Technical Support
投稿日: 2021-07-12

Has anyone used this AB for IHC in rat brain tissue? 

質問者: 匿名
Thank you for your question. We do not have publications specifically in rat brain tissue, but we have many publication in other rat tissues such as kidney, intesting and heart. We do have publications in mouse brain samples, so I would expect it to work perfectly fine in rat brain as well.
回答者: Tech Service
投稿日: 2021-06-01

Hi - I see that this antibody can detect C3 (which has a molecular weight of 150kDa), but that it is also recommended to detect C3a and C3b. How do I specifically probe for C3a and C3b separately on a western blot? Do I add in something to break C3 down?

質問者: C3question
Thank you for your question. At this time, we are not able to provide any specific inductions or treatments for samples to increase expression of the different C3 subunits. This is a research-based question, and we would recommend you consult with the primary literature. There are many product citations using this antibody for Western blotting applications, so it may be useful to start by reviewing the citations on our website for this antibody.
回答者: Technical Support
投稿日: 2019-07-12

Has this antibody specificity been validated? If so, can you include the link to evidence/publication? Thank you.

質問者: Shandiz
Thank you for your question. This antibody has many data images published on this product page, all showing validation. It also has many citations showing it’s use in publications. You can find them here: https://www.scbt.com/scbt/misc/citationResults.jsp ?_requestid=6883845
回答者: Technical Support
投稿日: 2019-05-29

Under what conditions have you demonstrated a positive C3 signal in human paraffin samples? I have tried numerous epitope retrieval protocols and seen nothing. I'm disappointed that you discontinued the goat polyclonal V-20 in favor of this product.

質問者: squareshot
Thank you for your question. We are sorry to hear that this antibody is not performing for you as expected. We want you to be successful in your research! We use a 1:50 dilution with this antibody on paraffin sections, after a antigen retrieval in sodium citrate with heat. Please contact our Technical Service Department by phone, e-mail or live chat. We are more than happy to help you troubleshoot the antibody. Also, we do warranty our antibodies for one year from the date of purchase, so if we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge, or issue a credit or refund if that is not possible.
回答者: Tech Service
投稿日: 2017-04-12
  • y_2025, m_8, d_27, h_5CST
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によって、 5つ星のうち 5と評価されています。 Works well4% PFA fixed, frozen section, rat cochlea, IF microscopy. Worked well.
投稿日: 2018-08-01
によって、 5つ星のうち 1と評価されています。 Does not detect human C3 in paraffin tissuesI tried using this antibody to detect human C3 in paraffin-embedded tissues, using three different antigen retrieval techniques, and saw nothing. I also tried using the unconjugated antibody followed by an anti-mouse secondary, saw nothing. On the other hand, the discontinued goat polyclonal worked.
投稿日: 2017-05-08
によって、 5つ星のうち 5と評価されています。 Great for all manner of C3 detection.This is a proven winner in C3 detection. It has broad range or reactivity and great specificity. It will provide you the results and signal you want in all standard applications. It has been published with more than 10 times.
投稿日: 2017-01-05
によって、 5つ星のうち 5と評価されています。 Superb!!!Great western if and ihc results; strong signal and low background
投稿日: 2017-01-05
によって、 5つ星のうち 4と評価されています。 IHC-Psome macrophages were positive for C3 by this antobody in IHC-P method
投稿日: 2016-12-30
によって、 5つ星のうち 5と評価されています。 Great in multiple applicationsThis antibody works very well in Western Blot, IF and IHC(P)
投稿日: 2016-12-23
によって、 5つ星のうち 5と評価されています。 I used it to detect mouse complement C3bI used it to detect mouse complement C3b with western blot. I should say that it is a wonderful product which gives a clear and concise band without obvious mixed band. I do have bought many of your product (mainly antibodies), they all works well, and the price is acceptable. You helped me a lot in my experiments, and I will keep on using your product.
投稿日: 2015-10-15
によって、 5つ星のうち 5と評価されています。 Great stain in mouse intestinal tissuesGreat stain in mouse intestinal tissues.
投稿日: 2015-08-06
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C3抗体(B-9)は、 16件のレビューによって、 5つ星のうち 4.7と 評価されています。
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