Bcl-w CRISPR/Cas9 KO Plasmid (h): sc-402639

Overview

BCL2L2 encodes Bcl-w, an anti-apoptotic member of the BCL-2 family that preserves mitochondrial outer membrane integrity by restraining pro-death effectors such as BAX and BAK. Through control of mitochondrial apoptosis, Bcl-w influences intrinsic cell death decisions in response to growth factor withdrawal, DNA damage, and oxidative stress, shaping cell survival, stress adaptation, and tissue homeostasis. BCL2L2 activity intersects with pathways regulating mitochondrial dynamics and survival signaling, including PI3K/AKT- and MAPK-associated inputs that converge on BCL-2 family balance. Dysregulated expression of Bcl-w has been associated with altered apoptotic thresholds and survival phenotypes relevant to oncogenesis and treatment response in multiple tumor contexts.

Bcl-w CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BCL2L2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BCL2L2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BCL2L2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Bcl-w protein expression.

This CRISPR knockout system enables efficient generation of BCL2L2-deficient cell models for investigation of Bcl-w signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting BCL2L2 exon(s) critical for Bcl-w function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple BCL2L2 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Bcl-w CRISPR/Cas9 KO Plasmid (h) and Bcl-w CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BCL2L2 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Bcl-w HDR Plasmid (h) and Bcl-w HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BCL2L2 homology arms to support homology-directed repair at defined BCL2L2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.