BAF CRISPR/Cas9 KO Plasmid (h): sc-401654
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BAF CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BAF genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BAF Antibody (A-11): sc-166324
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BAF CRISPR/Cas9 KO Plasmid (h) | sc-401654 | 20 µg | $397.00 |
Overview
BANF1 encodes barrier-to-autointegration factor (BAF), a conserved DNA-binding protein that bridges double-stranded DNA to LEM-domain nuclear envelope proteins and supports chromatin organization. BAF is essential for post-mitotic nuclear envelope reassembly, regulation of higher-order chromatin architecture, and maintenance of genome stability during the cell cycle. Through its interactions with lamins, emerin, and other nuclear lamina components, BAF influences transcriptional programs linked to nuclear structure and mechanotransduction. Dysregulation of BANF1/BAF has been associated with nuclear envelopathies and progeroid phenotypes, making it relevant for studying disease mechanisms tied to nuclear integrity and chromatin misregulation.
BAF CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BANF1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BANF1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BANF1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BAF protein expression.
This CRISPR knockout system enables efficient generation of BANF1-deficient cell models for investigation of BAF signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BANF1 exon(s) critical for BAF function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BANF1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BAF CRISPR/Cas9 KO Plasmid (h) and BAF CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BANF1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BAF HDR Plasmid (h) and BAF HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BANF1 homology arms to support homology-directed repair at defined BANF1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.