Ape2 CRISPR/Cas9 KO Plasmid (h): sc-407307
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ape2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ape2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ape2 CRISPR/Cas9 KO Plasmid (h) | sc-407307 | 20 µg | $397.00 |
Overview
Human APEX2 encodes apurinic/apyrimidinic endonuclease 2 (Ape2), a nuclease that recognizes abasic sites and participates in DNA base excision repair and genome maintenance. Ape2 contributes to the processing of damaged DNA termini and supports cellular responses to oxidative and replication-associated lesions, linking its activity to replication stress management and checkpoint signaling. By influencing the accumulation and resolution of DNA damage, APEX2 function is relevant to mechanisms that underlie mutagenesis and chromosomal instability observed in cancer and other disorders associated with impaired DNA repair capacity. As a result, APEX2 is frequently studied in contexts such as DNA damage response crosstalk, oxidative stress biology, and repair pathway choice.
Ape2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the APEX2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the APEX2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the APEX2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ape2 protein expression.
This CRISPR knockout system enables efficient generation of APEX2-deficient cell models for investigation of Ape2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting APEX2 exon(s) critical for Ape2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple APEX2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ape2 CRISPR/Cas9 KO Plasmid (h) and Ape2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the APEX2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ape2 HDR Plasmid (h) and Ape2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by APEX2 homology arms to support homology-directed repair at defined APEX2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.