Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
AMID/AIFM2/FSP1 CRISPR/Cas9 KO Plasmid (h): sc-406251
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- AMID/AIFM2/FSP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the AMID/AIFM2/FSP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: AMID/AIFM2/FSP1 Antibody (B-6): sc-377120
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AMID/AIFM2/FSP1 CRISPR/Cas9 KO Plasmid (h) | sc-406251 | 20 µg | $397.00 |
Overview
AIFM2, also known as AMID/FSP1, encodes a flavoprotein oxidoreductase implicated in redox homeostasis and mitochondrial-associated cell death signaling. FSP1 functions as a NAD(P)H-dependent CoQ10 oxidoreductase at membranes, providing a glutathione-independent defense against lipid peroxidation and suppressing ferroptotic cell death. Through its links to reactive oxygen species control, membrane lipid integrity, and apoptotic/mitochondrial stress responses, AMID/AIFM2 is frequently studied in oxidative stress biology and metabolic adaptation. Dysregulated AIFM2/FSP1 activity has been associated with contexts of therapy resistance and tumor cell survival, making it relevant for mechanistic studies of cell fate decisions under stress.
AMID/AIFM2/FSP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the AIFM2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the AIFM2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the AIFM2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish AMID/AIFM2/FSP1 protein expression.
This CRISPR knockout system enables efficient generation of AIFM2-deficient cell models for investigation of AMID/AIFM2/FSP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting AIFM2 exon(s) critical for AMID/AIFM2/FSP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple AIFM2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by AMID/AIFM2/FSP1 CRISPR/Cas9 KO Plasmid (h) and AMID/AIFM2/FSP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the AIFM2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by AMID/AIFM2/FSP1 HDR Plasmid (h) and AMID/AIFM2/FSP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by AIFM2 homology arms to support homology-directed repair at defined AIFM2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.