Aldehyde dehydrogenase 3-A1/ALDH3A1 CRISPR/Cas9 KO Plasmid (m): sc-419080

Overview

Aldh3a1 encodes aldehyde dehydrogenase 3-A1 (ALDH3A1), an NAD(P)+-dependent enzyme that oxidizes reactive aliphatic and aromatic aldehydes generated during lipid peroxidation and xenobiotic metabolism. By detoxifying electrophilic aldehydes and contributing to redox homeostasis, ALDH3A1 supports cellular defenses against oxidative and carbonyl stress and intersects with glutathione-linked stress response pathways. In mouse tissues, ALDH3A1 activity has been associated with protection of epithelial compartments exposed to environmental and metabolic insults, informing studies of inflammation, aging-related stress, and carcinogenesis where aldehyde burden is elevated. Altered ALDH family function is frequently examined in models of metabolic dysfunction and tumor biology due to its impact on detoxification capacity and cellular stress tolerance.

Aldehyde dehydrogenase 3-A1/ALDH3A1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Aldh3a1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Aldh3a1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Aldh3a1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Aldehyde dehydrogenase 3-A1/ALDH3A1 protein expression.

This CRISPR knockout system enables efficient generation of Aldh3a1-deficient cell models for investigation of Aldehyde dehydrogenase 3-A1/ALDH3A1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Aldh3a1 exon(s) critical for Aldehyde dehydrogenase 3-A1/ALDH3A1 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Aldh3a1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by Aldehyde dehydrogenase 3-A1/ALDH3A1 CRISPR/Cas9 KO Plasmid (m) and Aldehyde dehydrogenase 3-A1/ALDH3A1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Aldh3a1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by Aldehyde dehydrogenase 3-A1/ALDH3A1 HDR Plasmid (m) and Aldehyde dehydrogenase 3-A1/ALDH3A1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Aldh3a1 homology arms to support homology-directed repair at defined Aldh3a1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.