Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
adseverin CRISPR/Cas9 KO Plasmid (m): sc-422815
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- adseverin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the adseverin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: adseverin Antibody (C-2): sc-376136
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
adseverin CRISPR/Cas9 KO Plasmid (m) | sc-422815 | 20 µg | $397.00 |
Overview
Scin encodes adseverin, a Ca²⁺-regulated actin-severing and capping protein of the gelsolin superfamily that remodels filamentous actin dynamics in a stimulus-dependent manner. By promoting rapid actin filament disassembly and reorganization, adseverin supports processes such as membrane trafficking, cell shape changes, and regulated secretion, particularly in hematopoietic and secretory cell contexts. Its activity intersects with calcium signaling and cytoskeletal control pathways that influence adhesion, migration, and vesicle exocytosis. Dysregulated actin remodeling and calcium-dependent secretion are frequently implicated in inflammatory and neoplastic phenotypes, making Scin a useful node for mechanistic studies of cytoskeleton-linked disease biology in mouse models.
adseverin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Scin gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Scin together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Scin open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish adseverin protein expression.
This CRISPR knockout system enables efficient generation of Scin-deficient cell models for investigation of adseverin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Scin exon(s) critical for adseverin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Scin genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by adseverin CRISPR/Cas9 KO Plasmid (m) and adseverin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Scin locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by adseverin HDR Plasmid (m) and adseverin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Scin homology arms to support homology-directed repair at defined Scin target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.