Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
γ Enolase CRISPR/Cas9 KO Plasmid (m): sc-420179
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- γ Enolase CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the γ Enolase genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: γ Enolase Antibody (D-7): sc-376375
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
γ Enolase CRISPR/Cas9 KO Plasmid (m) | sc-420179 | 20 µg | $397.00 |
Overview
Eno2 encodes γ-enolase (neuron-specific enolase), a glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate and supports neuronal energy metabolism. In mouse, γ-enolase is enriched in neurons and neuroendocrine tissues and links central carbon metabolism with cellular programs involved in differentiation, survival, and stress adaptation. Altered ENO2/γ-enolase expression is widely used as a marker of neuronal and neuroendocrine cell state and has been associated with mechanisms relevant to neurodegeneration, hypoxic/ischemic injury, and tumor metabolism. These features make Eno2 a useful entry point for studying metabolic rewiring and cell-type–specific regulation in the nervous system.
γ Enolase CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Eno2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Eno2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Eno2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish γ Enolase protein expression.
This CRISPR knockout system enables efficient generation of Eno2-deficient cell models for investigation of γ Enolase signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Eno2 exon(s) critical for γ Enolase function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Eno2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by γ Enolase CRISPR/Cas9 KO Plasmid (m) and γ Enolase CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Eno2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by γ Enolase HDR Plasmid (m) and γ Enolase HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Eno2 homology arms to support homology-directed repair at defined Eno2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.